摘要
利用基因组重测序技术对江汉鸡群体卵泡雌激素(FSH)β亚基基因序列进行全面筛选,结果表明,该群体内一共发现35个SNPs和2个indels,其中cds区1个SNP突变造成甘氨酸变成丝氨酸。结合生物信息学软件分析基因保守序列,预测M6A修饰位点、调控元件以及miRNA靶向序列,结果发现7个SNPs位于潜在的调控元件序列中,7个SNPs和1个indel位于潜在miRNA结合序列中。所筛选的上述遗传变异位点可能参与了FSHβ基因的表达调控,在江汉鸡繁殖特性中扮演一定的角色。
In this study,the genome-wide re-sequencing technology was applied in Jianghan chicken population for genetic variation identification,the results showed that,35 single nucleotide polymorphisms(SNPs)and 2 indels in theβsubunit gene of follicular estrogen(FSH)were found,one single-base mutation in the cds could cause glycine to serine.Combining with bioinformatics softwares,M6A modification site prediction,regulatory element prediction and miRNA targeting sequence prediction were further analyzed.It was found that 7 single-base mutations were located in the potential regulatory element sequence,and 7 single-base mutations and 1 indel were located in the potential miRNA binding sequence.Those genetic mutations may be involved in the regulation of FSHβgene expression,and play a certain role in the genetic characteristics of Jianghan chicken.
作者
黄涛
潘爱銮
申杰
吴艳
蒲跃进
张昊
孙静
梁振华
杜金平
朱昌友
皮劲松
HUANG Tao;PAN Ai-luan;SHEN Jie;WU Yan;PU Yue-jin;ZHANG Hao;SUN Jing;LIANG Zhen-hua;DU Jin-ping;ZHU Chang-you;PI Jin-song(Institute of Animal Husbandry and Vetervinary,Hubei Academy of Agricultural Sciences/Hubei Innovation Center of Agricultural Science and Technology/Hubei Key Lab of Animal Embryo Technology and Molecular Breeding,Wuhan 430064,China;Hubei Animal Husbandry Breeding Farm,Wuhan 434010,China)
出处
《湖北农业科学》
2021年第23期127-130,共4页
Hubei Agricultural Sciences
基金
湖北省畜禽优质增效与绿色养殖技术创新行动(2018skjcx05)
湖北省农业科学院青年基金项目(2020NKYJJ11)。
作者简介
黄涛(1989-),男,河南光山人,助理研究员,博士,主要从事家禽遗传育种研究,(电话)13100655209(电子信箱)huangtao214@126.com;通信作者:皮劲松,研究员,主要从事家禽育种与养殖的研究,(电话)13886171120(电子信箱)pijin-song@sina.com。
