摘要
目的研究枸杞多糖(LBP)对小鼠GC-1spg精原细胞氧化损伤的保护作用,探讨其治疗男性不育症的作用及机制。方法通过次黄嘌呤-黄嘌呤(HX-XO)氧化酶体系建立体外小鼠GC-1spg精原细胞氧化应激模型,分别以50、100、200μg·mL^(-1)的LBP为低、中、高剂量设定给药组,另设空白组和模型组,培养24 h,MTT比色法测定细胞活力,通过测定细胞内丙二醛(MDA)的生成量、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性、谷胱甘肽(GSH)水平评价LBP对小鼠GC-1spg精原细胞氧化损伤的保护作用,通过检测细胞中Caspase-3和Caspase-9的活性,评价LBP对小鼠GC-1spg精原细胞凋亡的影响。结果不同浓度的LBP可以显著改善HX-XO诱导的小鼠GC-1spg精原细胞氧化损伤,提高细胞活性,降低MDA生成量,提高SDO、CAT活性和GSH水平,降低Caspase-3和Caspase-9的活性,抑制细胞凋亡。结论LBP可通过抗氧化作用对小鼠GC-1spg精原细胞氧化损伤起保护作用,改善细胞凋亡,提高细胞活力,可作为改善男性不育症的潜力药物。
OBJECTIVE To study the protective effect of Lycium barbarum polysaccharide(LBP)on oxidative damage of GC-1spg spermatogonia in mice,and to explore its therapeutic effect and mechanism on male infertility.METHODS The oxidative stress model of GC-1spg spermatogonia was established by hypoxanthine xanthine(HX-XO)oxidase system in vitro.LBP(50,100,200μg·mL^(-1))was used as low,medium,and high dose respectively.Control group and model group were set up and cultured for 24 h,MTT assay was used to determine the cell viability.The protective effect of LBP on oxidative damage of GC-1spg spermatogonia was evaluated by measuring the production of MDA,the activities of superoxide dismutase(SOD)and catalase(CAT),and the level of glutathione(GSH).The effects of LBP on apoptosis of GC-1spg spermatogonia were evaluated by detecting the activities of Caspase-3 and Caspase-9.RESULTS LBP at different concentrations could significantly ameliorate the oxidative damage of GC-1spg spermatogonia induced by HX-XO,increase the cell activity,reduce the production of MDA,increase the activities of SOD,CAT and GSH,decrease the activities of Caspase-3 and caspase-9,and inhibit apoptosis.CONCLUSION LBP can protect GC-1spg spermatogonial cells from oxidative damage and inhibit cell apoptosis through antioxidation,which can be used as a potential drug to improve male infertility.
作者
徐杨超
XU Yang-chao(Jiangxi Medical College,Shangrao 314000,China)
出处
《海峡药学》
2021年第11期10-14,共5页
Strait Pharmaceutical Journal
基金
2019年度江西省卫生健康委科技计划(指导性计划)项目表,编号20195662。
关键词
枸杞多糖
GC-1spg精原细胞
抗氧化
细胞活力
Lycium barbarum polysaccharides
GC-1spg spermatogonia
Antioxidation
Cell viability
