摘要
目的:观察醒脑静注射液对大鼠脑缺血急性期半暗带转化的影响。方法:采用线栓法制备大鼠大脑中动脉闭塞模型。除外假手术组,其余各组造模成功后随机分为模型组和醒脑静组。醒脑静组按0.18 mL/100 g腹腔注射醒脑静注射液,假手术组和模型组分别给予等量生理盐水。术后24 h,尼氏染色观察大鼠半暗带区神经元形态变化,透射电子显微镜观察神经血管单元(neurovascular unit,NVU)超微结构变化,原位细胞凋亡法检测(TdT-mediated dutp nick-end labeling,TUNEL)观察大鼠缺血半暗带区细胞凋亡情况,磁共振分别于4.5 h和24 h观察大鼠不同时间段半暗带区缺血演变过程。结果:与假手术组相比,模型组神经元数量明显减少,尼氏小体结构破坏崩裂,轮廓模糊或消失;透射电镜观察可见NVU超微结构病理形态明显损伤;模型组TUNEL染色可见大量的凋亡细胞(P<0.01);磁共振结果可见模型组大鼠脑组织存在大面积梗死。与模型组相比,醒脑静组神经元和NVU超微结构病理形态明显改善;凋亡细胞数量明显减少(P<0.01),半暗带丢失率明显降低(P<0.05)。结论:醒脑静注射液能够改善缺血半暗带区神经元状态,减少胶质细胞和微血管损伤,抑制细胞凋亡,在一定程度上挽救了部分半暗带,对脑缺血急性期半暗带区脑组织具有一定的保护作用。
Objective:To observe the effect of Xingnaojing injection on penumbra transformation in rats with acute cerebral ischemia.Methods:The rat model of middle cerebral artery occlusion was established by suture occlusion.Except the sham operation group,the other groups were randomly divided into the model group and the Xingnaojing group.The rats in the Xingnaojing group were intraperitoneally injected with Xingnaojing injection at 0.18 mL/100 g,and the sham operation group and model group were given the same amount of normal saline,respectively.Twenty four hours after the establishment of the model,the morphological changes of neurons in the penumbra of the rats were observed by Nissl staining,the ultrastructural changes of neurovascular unit(Neurovascular unit,NVU)were observed by transmission electron microscope,and the apoptosis of the ischemic penumbra was detected by in situ apoptosis(TdT-mediated Dutp Nick-End Labeling,TUNEL).Magnetic resonance imaging was used to observe the ischemic evolution of the penumbra of the same rat at 4.5 h and 24 h,respectively.Results:Compared with the sham operation group,the number of neurons in the model group was significantly reduced,the structure of Nissl corpuscles was destroyed,the outline was blurred or disappeared,the pathological morphology of NVU ultrastructure was obviously damaged under transmission electron microscope,a large number of apoptotic cells could be seen in the model group by TUNEL staining(P<0.01),and magnetic resonance imaging showed that there was a large area infarction in the brain tissue of the model group.Compared with the model group,the pathomorphology of neurons and NVU ultrastructure in the Xingnaojing group was significantly improved,the number of apoptotic cells was significantly decreased(P<0.01),and the loss rate of penumbra was significantly lower in the Xingnaojing group(P<0.05).Conclusions:Xingnaojing injection can improve the state of neurons in ischemic penumbra,reduce the injury of glial cells and microvessels,inhibit apoptosis,promote the transformation of penumbra in rats with acute cerebral ischemia,and save part of penumbra to some extent.It has a certain protective effect on the brain tissue of penumbra in the acute stage of cerebral ischemia.
作者
孙逸坤
吴丽君
李媛媛
刘浩琦
安娜
宋珂
张华
高永红
SUN Yi-kun;WU Li-jun;LI Yuan-yuan;LIU Hao-qi;AN Na;SONG Ke;ZHANG Hua;GAO Yong-hong(Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing,Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine,Beijing 100700,China;Institute for Brain Disorders,Beijing University of Chinese Medicine,Beijing 100029,China;Traditional Chinese Medicine Hospital of Weishi County,Kaifeng 475500,China)
出处
《海南医学院学报》
CAS
2021年第23期1761-1767,共7页
Journal of Hainan Medical University
基金
国家自然科学基金项目(81673899)。
关键词
醒脑静注射液
脑缺血急性期
半暗带
神经元
核磁
Xingnaojing injection
Acute stage of cerebral ischemia
Penumbra
Neuron
MRI
作者简介
孙逸坤,E-mail:360841479@qq.com;通讯作者:张华,主任医师,博士,E-mail:nwkzhhsf@aliyun.com;通讯作者:高永红,研究员,博士,E-mail:gaoyh7088@163.com。
