摘要
【目的】明确低温胁迫对新菠萝灰粉蚧致死效果的影响。【方法】观察新菠萝灰粉蚧不同发育阶段虫态在不同低温(0、4、10℃)下5个不同处理时间(1、2、4、6、8 h)的致死情况,以常温26℃为对照,分析低温胁迫对该粉蚧死亡率的影响。【结果】0、4、10℃低温处理对新菠萝灰粉蚧一龄若虫和二龄若虫死亡率有显著影响,各处理时间的死亡率均大于30%;二龄若虫经0、4℃低温处理6、8 h后死亡率高达95%以上;10℃低温处理1、2、4 h后三龄若虫死亡率均低于10%,与常温26℃对照差异不显著。未成熟雌成虫在10℃低温处理4、6 h后死亡率与常温26℃对照差异不显著。对于成熟雌成虫,10℃低温不同处理时间的死亡率均小于3%,与常温26℃对照差异不显著。【结论】表明低温胁迫对新菠萝灰粉蚧一龄若虫和二龄若虫的死亡率有显著影响,三龄及三龄后若虫在10℃能保持较好的生存能力。
【Objective】The effect of low temperature stress on lethality of Dysmicoccus neobrevipes Beardsley(Hemiptera:Pseudococcidae)was determined.【Method】The lethality of D.neobrevipes at different development stages under different low temperatures(0℃,4℃,10℃)for five different time treatments(1 h,2 h,4 h,6 h,8 h)was observed in this study,with the compared temperature of 26℃as control.And the effect of low temperature stress on the mortality of D.neobrevipes was analyzed.【Result】There were significant effects on the mortalities of the 1 st and 2 nd instar nymphs of D.neobrevipes under low temperature treatments of 0℃,4℃and 10℃,and the mortality in each treatment was more than 30%.The mortality of the 2 nd instar nymph of D.neobrevipes was over 95%after being treated for 6 h and 8 h at the low temperature of 0℃and 4℃.There were no significant differences in the mortality of the 3 rd instar nymph of D.neobrevipes after being treated for 1 h,2 h and 4 h at 10℃compared with that at 26℃,with the mortality of less than 10%.For immature female adults of D.neobrevipes,there were no significant differences in the mortalities after being treated for 4 h,6 h at 10℃compared with those at 26℃.For mature female adults,there were no significant differences in the mortalities of D.neobrevipes in different treatment times at 10℃(less than 3%)compared with those at 26℃.【Conclusion】The results indicated that the low temperature stress had a significant effect on the mortality of the 1 st and 2 nd instar nymphs of D.neobrevipes,and the development stage of D.neobrevipes after the 3 rd instar nymph could maintain a good survival ability at 10℃.
作者
李德伟
吴建辉
覃振强
LI Dewei;WU Jianhui;QIN Zhenqiang(Sugarcane Research Institute,Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Sugarcane Genetic Improvement/Guangxi Key Laboratory of Sugarcane Biotechnology and Genetic Improvement,Ministry of Agriculture and Rural Affairs,Nanning 530007,China;Guangdong Key Laboratory of Bio-Pesticide Innovation and Application/Engineering Technology Research Center of Pest Biocontrol,Ministry of Education,Guangzhou 510640,China)
出处
《广东农业科学》
CAS
2021年第11期103-111,共9页
Guangdong Agricultural Sciences
基金
国家自然科学基金(31760540)
广西农业科学院基本科研业务专项(桂农科2021YT013)。
关键词
检疫性有害生物
新菠萝灰粉蚧
剑麻害虫
低温胁迫
致死率
暴露试验
quarantine pest
Dysmicoccus neobrevipes Beardsley
sisal pest
low temperature stress
lethality rate
exposure experiment
作者简介
李德伟(1980-),男,硕士,副研究员,研究方向为昆虫生物学及害虫防治,E-mail:ldw11023@163.com;通信作者:覃振强(1975-),男,博士,副研究员,研究方向为昆虫生物学及农药学,E-mail:qinzqcn@163.com。
