摘要
目的观察癫痫患儿外周血血清中泛素羧基末端水解酶L1(UCH-L1)、S100B蛋白表达水平并探讨其临床意义。方法选择河北省唐山市妇幼保健院(以下简称“我院”)2018年10月至2019年10月初诊的,且入院前24 h内有癫痫发作的癫痫患儿80例为病例组,选择同期在我院健康体检的儿童80名为对照组。采集清晨空腹静脉血,双抗体夹心酶联免疫吸附试验检测血清UCH-L1、S100B蛋白的表达水平,并比较病例组中不同癫痫发作持续时间、不同癫痫发作类型的患儿血清UCH-L1、S100B蛋白的表达水平。结果病例组UCH-L1与S100B蛋白水平显著高于对照组(P<0.01)。癫痫持续时间≥5 min组UCH-1、S100B蛋白水平高于癫痫持续时间<5 min组(P<0.05或P<0.01)。全面性发作组UCH-1、S100B蛋白水平显著高于局灶性发作组(P<0.01)。结论癫痫患儿外周血中UCH-L1、S100B蛋白水平升高,且二者的增高与癫痫发作持续时间和发作类型有一定关系。
Objective To observe the expression levels of serum ubiquitin carboxy-terminal hydrolase-l1(UCH-L1)and S100B protein in peripheral blood of children with epilepsy and to explore its significance.Methods A total of 80 children with epilepsy who were newly diagnosed in Tangshan Maternal and Child Health Hospital of Hebei Province(hereinafter referred to as“our hospital”)from October 2018 to October 2019 and had seizure within 24 hours before admission were selected as the case group,and a total of 80 healthy children in our hospital were selected as the control group.Fasting venous blood was collected in the morning,and the serum UCH-L1 and S100B protein expression levels were detected by double-antibody sandwich enzyme-linked immunosorbent assay,and the serum UCH-L1 and S100B protein expression levels were compared among the children with different seizure duration and seizure types in the case group.Results UCH-L1 and S100B protein levels in the case group were significantly higher than those in the control group(P<0.01).UCH-L1 and S100B protein levels in epilepsy duration≥5 min group were higher than those in epilepsy duration<5 min group(P<0.05 or P<0.01).UCH-L1 and S100B protein levels in generalized seizure group were significantly higher than those in focal seizure group(P<0.01).Conclusion UCH-L1 and S100B protein levels in the peripheral blood of children with epilepsy are increased,and the increase of both is related to the duration and type of epileptic seizure.
作者
张双
闫凯
李树华
张静
贾玉凤
ZHANG Shuang;YAN Kai;LI Shuhua;ZHANG Jing;JIA Yufeng(Department of Paediatrics,Tangshan Maternal and Child Health Care Hospital,Hebei Province,Tangshan,063000,China)
出处
《中国医药导报》
CAS
2021年第32期104-107,共4页
China Medical Herald
基金
河北省医学科学研究重点课题计划项目(20181321)。
作者简介
张双(1970.1-),女,硕士,主任医师,硕士生导师,研究方向:小儿神经。
