摘要
目的:探讨献血者捐献单采血小板贮存期间血小板扩散功能的变化。方法:2017年8月到2020年6月选择在本血站参加单采血小板献血的献血者66例作为研究对象,每个献血者的单采血小板血液样本分为3管-A管、B管与C管,将产品置于血小板振荡保存箱22℃振荡保存,分别在贮存第1 d(A管)、第3 d(B管)、第5 d(C管),检测血小板扩散功能的变化情况。结果:A管、B管与C管的Hct(Hematocrit,红细胞压积)、Hb(Hemoglobin,血红蛋白)、PDW(Platelet distribution width,血小板分布宽度)、WBC(leukocyte,白细胞)、RBC(Red blood cell,红细胞)、血小板(Platelets,PLT)、MPV(Mean platelet volume,平均血小板体积)对比差异无统计学意义(P>0.05),B管与C管的P-LCR(platelet-larger cell ratio,大型血小板比例)值与血清白介素(Interleukin,IL)-1β、IL-6、肿瘤坏死因子(Tumor necrosis factor,TNF)-α含量高于A管(P<0.05),C管高于B管(P<0.05)。B管与C管的血小板膜糖蛋白CD62p表达水平高于A管(P<0.05),血小板最大聚集率低于A管(P<0.05),C管与B管对比差异也都有统计学意义(P<0.05)。A管、B管与C管的扩散后血小板合格率分别为97.0%、86.4%、77.3%,三组间对比差异有统计学意义(P<0.05)。结论:献血者捐献单采血小板贮存时间可影响血小板扩散功能,导致炎症细胞因子释放量增加,可降低血小板血小板最大聚集率,提高血小板膜糖蛋白CD62p表达水平,从而降低输注疗效。
Objective:To investigate the changes of platelet diffusion function during storage of apheresis platelets from blood donors.Methods:A total of 66 blood donors,who participated in platelet apheresis blood donation at blood centers in the region from August 2017 to June 2020,were selected as the research subjects.The platelet apheresis blood sample of each case was divided into 3 tubes-A tube,B tube and C tube,which were stored in a platelet incubator at 20℃,tube A 1 d after storage,tube B 3 d after storage,and tube C 5 d after storage were detected platelet diffusion functional changes.Results:There were not significantly differences in the Hct(Hematocrit),Hb(Hemoglobin),PDW(Platelet distribution width),WBC(leukocyte),RBC(Red blood cell),Platelets(PLT),and MPV(Mean platelet volume)among tube A,tube B and tube C(P>0.05).The P-LCR(platelet-larger cell ratio),serum interleukin(IL)-1β,IL-6,and tumor necrosis factor(Tumor necrosis factor,TNF)-αcontent of tube B and tube C were higher than those of tube A(P<0.05),tube C were higher than tube B(P<0.05).The expression levels of platelet membrane glycoprotein CD62 p in tube B and tube C were higher than those in tube A(P<0.05),and the maximum platelet aggregation rate in tube B and tube C was lower than that in tube A(P<0.05),and the difference between tube C and tube B was also statistically significant(P<0.05).The platelet pass rate after diffusion of tube A,tube B and tube C were 97.0%,86.4%,and 77.3%,respectively,with statistically significant differences among the three groups(P<0.05).Conclusion:The storage time of apheresis platelets donated by blood donors may affect the platelet diffusion function,increase the release of inflammatory cytokines,can reduce the maximum platelet aggregation rate and increase the expression level of platelet membrane glycoprotein CD62 p,thus reducing the efficacy of infusion.
作者
张文萍
李东辉
党璇
党娥
张小乐
ZHANG Wen-ping;LI Dong-hui;DANG Xuan;DANG E;ZHANG Xiao-le(Department of Blood Transfusion,First Affiliated Hospital of Air Force Military Medical University,Xi'an,Shaanxi,710032,China;Department of Oncology,Shaanxi Provincial People's Hospital,Xi'an,Shaanxi,710068,China)
出处
《现代生物医学进展》
CAS
2021年第18期3486-3489,3476,共5页
Progress in Modern Biomedicine
基金
陕西省重点研发计划项目(2018SF-221)。
关键词
献血者
单采血小板
贮存时间
扩散功能
炎症因子
凝血功能
大型血小板比例
Blood donors
Apheresis platelet
Storage time
Diffusion function
Inflammatory factor
Coagulation function
Platelet-larger cell ratio
作者简介
张文萍(1984-),女,本科,技师,研究方向:输血科,电话:15991779150,E-mail:ping1221888@163.com;通讯作者:李东辉,女,硕士研究生,主任医师,研究方向:肿瘤内科,电话:13709251030,E-mail:ping1221888@163.com。
