摘要
目的观察喂养不耐受(FI)极早产儿胎粪中的肠道菌群变化,分析其肠道菌群特征。方法极早产儿24例,根据喂养耐受情况分为喂养耐受(FT)组8例和FI组16例,均采集出生时的胎粪,采用高通量测序技术检测粪便肠道菌群,测算两组患儿肠道菌群chao1指数、shannon指数,观察肠道细菌的相对丰度。结果FI组、FT组chao 1指数分别为1538.56±545.72、1052.50±106.86,P<0.05。FI组、FT组shannon指数分别为7.06±1.51、5.62±1.37,P<0.05。与FT组比较,FI组肠道蓝藻菌门(Cyanobacteria)、酸杆菌门(Acidobacteria)、疣微菌门(Verrucomicro⁃bia)、绿弯菌门(Chloroflexi)、未分类的蓝藻菌科(unidentified_Cyanobacteria)、未分类的蓝藻菌属(unidentified_Cyano⁃bacteria)、假单胞菌属(Pseudomonas)、小链球菌属(Catellicoccus)、未分类的毛螺菌属(unidentified_Lachnospiraceae)、马赛菌属(Massilia)和纤维单胞菌属(Cellulomonas)相对丰度升高,海洋杆菌属(Oceanobacter)相对丰度降低(z分别为-2.572,-2.267,-3.307,-2.664,-2.572,-2.572,-2.205,-2.233,-2.347,-2.728,-2.770,-2.053;P均<0.05)。结论FI的极早产儿出生时肠道菌群的多样性下降,Cyanobacteria、Cellulomonas、Pseudomonas、Massilia等菌群富集明显。Cyanobacteria、Cellulomonas、Pseudomonas、Massilia等条件致病菌的菌群变化可能是极早产儿出现FI的原因。
Objective To observe the gut microbiota in meconium of extremely preterm infants with feeding intolerance(FI)and to analyze the microbial characteristics.Methods Twenty-four extremely premature infants’feces were collected at birth and were divided into two groups according to feeding tolerance(FT)or not:FT group(n=8)or FI group(n=16).The fecal microbiota was detected by high-throughput sequencing technology.Chao1 index and shannon index were measured,as well as the relative abundance of gut microbiota was observed.Results The chao 1 indices in the FI group and FT group were 1538.56±545.72 and 1052.50±106.86(P<0.05).The Shannon indices of FI group and FT group were 7.06±1.51 and 5.62±1.37(P<0.05).Compared with FT group,the relative abundances of Cyanobacteria,Acidobacteria,Verrucomicrobia,Chloroflexi,unidentified_Cyanobacteria(family),unidentified_Cyanobacteria(genus),Pseudomonas,Catellicoccus,unidentified_Lachnospiraceae,Massilia and Cellulomonas in the FI group increased,while that of Oceanobacter decreased(Z=-2.572,-2.267,-3.307,-2.664,-2.572,-2.572,-2.205,-2.233,-2.347,-2.728,-2.770,and-2.053;all P<0.05).Conclusion The diversity of gut microbiota in meconium of extremely premature infants with FI decreases,and Cyanobacteria,Cellulomonas,Pseudomonas,Massilia and other conditional pathogens are significantly enriched,which may be the cause of FI in extremely premature infants.
作者
罗梅娟
胡家奇
魏颖
欧巧群
翁志媛
王丽娜
张又祥
LUO Meijuan;HU Jiaqi;WEI Ying;OU Qiaoqun;WENG Zhiyuan;WANG Lina;ZHANG Youxiang(Department of Pediatrics,Guangzhou First People's Hospital,The Second Hospital Affiliated to South China University of Technology School of Medicine,Guangzhou 510180,China)
出处
《山东医药》
CAS
2021年第30期19-23,共5页
Shandong Medical Journal
基金
广东省科技计划资助项目(2017ZC0316)
广州市医药卫生科技一般引导项目(20171A011244)。
关键词
肠道菌群
喂养不耐受
极早产儿
早产儿
gut microbiota
feeding intolerance
extremely premature infants
premature infants
作者简介
第一作者:罗梅娟(1984-),女,硕士研究生,主治医师,主要研究方向为新生儿肠道微生态。E-mail:271219312@99.com;通信作者:张又祥(1963-),男,硕士研究生,主任医师,主要研究方向为先天性肥厚性幽门狭窄。E-mail:youxiangz@163.com。
