摘要
采用气相离子迁移谱(gas chromatography-ion mobility spectrometry,GC-IMS)技术对来源于重庆三峡库区的油菜花、五倍子花、枇杷花和柑橘花的4种特色中华蜜蜂蜂蜜(中蜂蜜)的挥发性有机成分进行测定和分析,建立不同植物来源中蜂蜜的判别模型,并对不同植物来源蜂蜜进行鉴别和分类。利用二维差谱法筛选出58个有效特征成分的特征峰作为表征中蜂蜜植物来源差异信息的特征变量,利用主成分分析(principal component analysis,PCA)和线性判别分析(linear discriminate analysis,LDA)方法建立判别模型。结果表明,该研究选取的特征变量经PCA处理后前3个主成分的累积贡献率为81.35%,得分图中4种中蜂蜜分布于不同的区域,无重叠或交叉,建立的判别模型可有效识别不同植物来源的中蜂蜜,准确率为100%,对其他植物来源中蜂蜜的误判率仅为5.9%。该研究利用GC-IMS测定中蜂蜜中的挥发性有机成分,结合PCA和LDA可以准确地区分重庆三峡库区不同蜜源植物特色的中蜂蜜样品,为中蜂蜜的品种鉴别和质量控制提供了新的技术参考。
The volatile organic compounds of four different plant sources honey of Apis cerana,rape flower honey,gallnut flower honey,loquat flower honey and orange blossom honey in the three Gorges Reservoir area of Chongqing,were determined and analyzed by gas chromatography-ion mobility spectrometry(GC-IMS).The discriminant model to identify and classify different plant sources honey was established.Two-dimensional differential analysis was used to screen out 58 effective characteristic peaks for the characterization of the different plant sources of honey of Apis cerana.A discriminant model was established by principal component analysis(PCA)and linear discriminant analysis(LDA).The results showed that the cumulative contribution rate of the top three principal components in this study was 81.35%,and the four kinds of honey of Apis cerana in the scores plot were distributed in different areas without overlapping or crossing.The established discriminant model could effectively identify the honey of Apis cerana from different plant sources,and the recognition accuracy was 100%.The misdiagnosis rate for honey of Apis cerana from other plant sources was only 5.9%.In this study,volatile components in honey of Apis cerana were determined by GC-IMS,the honey of Apis cerana samples of different plant sources in the three Gorges Reservoir area of Chongqing was accurately distinguished through PCA and LDA analysis,providing a new technical reference for the variety identification and quality control of honey of Apis cerana.
作者
刘振平
聂青玉
庞钶靖
张艳
姜容
LIU Zhenping;NIE Qingyu;PANG Kejing;ZHANG Yan;JIANG Rong(Chongqing Vocational Institute of Safety&Technology,Chongqing 404020,China;Chongqing Three Gorges Vocational College,Chongqing 404155,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2021年第22期273-278,共6页
Food and Fermentation Industries
基金
重庆市教育委员会科学技术研究计划青年项目资助项目(KJQN201804703)
重庆市教育委员会高校创新研究群体项目(CXQTP19037)。
关键词
气相离子迁移谱
重庆三峡库区
蜂蜜鉴别
主成分分析
线性判别分析
gas chromatography-ion mobility spectrometry
the three Gorges Reservoir area of Chongqing
honey identification
principal component analysis
linear discriminant analysis
作者简介
第一作者/共同通讯作者:刘振平,博士,副教授,E-mail:nping305@126.com;共同通讯作者:聂青玉,教授,E-mail:nqy318@163.com。
