摘要
为研究超富集植物青葙叶片生长和光合色素对锰胁迫的响应及其激素应答机制,采用不同浓度锰胁迫(0.5、30、60、100 mg/L)来进行水培试验,观察不同锰处理下青葙的生长状况,并测定其叶片生物量、叶面积、叶绿素以及内源激素的含量.结果显示:随着锰胁迫浓度的增加,青葙叶片的生物量、叶面积以及叶绿素和类胡萝卜素均存在显著差异(P<0.05),并且在30 mg/L处理组达到最大,然后随着锰浓度增加而降低.生长素(IAA)、赤霉素3(GA_(3))和玉米素(ZT)含量变化趋势一致,在30 mg/L时显著升高,与对照相比分别高出54.37%、51.62%、18.38%.茉莉酸(JA)和脱落酸(ABA)含量与锰浓度呈正相关,在100 mg/L时分别比对照高出164.11%、36.96%.本研究表明青葙叶片对锰胁迫的响应受到内源激素的调控,其中低浓度锰胁迫增加叶片中IAA、GA_(3)和ZT的含量,从而促进青葙的生长,而高浓度锰胁迫显著增加ABA和JA的含量,抑制其生长.
To study the responses of growth and photosynthetic pigments in the leaves of Celosia argentea Linn.to Mn stress and its regulation mechanism of endogenous hormones,hydroponics experiments were carried out under different concentrations of Mn stress(0.5,30,60,and 100 mg/L).C.argentea growth was then observed,and the leaf biomass,leaf area,chlorophyll,and endogenous hormone content were measured.Results showed that the biomass,leaf area,chlorophyll,and carotenoid were significantly different with the increase in Mn concentration(P<0.05),reached a maximum at 30 mg/L treatment group and then decreased with the increase in Mn concentration.The variation trends of auxin(IAA),Gibberellin 3(GA_(3)),and zeatin(ZT)were consistent and were significantly increased at 30 mg/L,which were 54.37%,51.62%,and 18.38%higher than those in the control group.The contents of jasmonic acid(JA)and abscisic acid(ABA)were positively correlated with the concentration of Mn stress;these were 164.11%and 36.96%at 100 mg/L higher than the control.These results indicate that the response of C.argentea leaves to Mn stress is regulated by endogenous hormones.Low concentrations of Mn stress significantly increased the content of IAA,GA_(3),and ZT in leaves,which promoted C.argentea growth.Under a high concentration of Mn stress,the content of ABA and JA increased significantly and inhibited its growth.
作者
雷玲
刘杰
蒋萍萍
朱真
丁志凡
蒋旭升
LEI Ling;LIU Jie;JIANG Pingping;ZHU Zhen;DING Zhifan;JIANG Xusheng(Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology,Guilin University of Technology,Guilin 541004,China;Technical Innovation Center of Mine Geological Environmental Restoration Engineering in Southern Karst Area of Ministry of National Resources,Nanning 530022,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2021年第5期1240-1245,共6页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(41867022)
广西高等学校高水平创新团队及卓越学者计划项目(桂财教函[2018]319号)资助。
关键词
锰胁迫
青葙
叶片
内源激素
光合色素
Mn stress
Celosia argentea Linn.
leaf
endogenous hormone
chlorophyll
作者简介
通讯作者:刘杰(E-mail:liujie@glut.edu.cn)。
