摘要
黄嘌呤氧化酶是尿酸生成的关键酶,通过抑制黄嘌呤氧化酶的活性可以有效降低机体内尿酸的含量。为探究黄芪多糖对黄嘌呤氧化酶的抑制作用,采用紫外分光光度法测定单位时间内酶促反应体系中酶活力的变化,计算不同质量浓度的黄芪多糖对黄嘌呤氧化酶的抑制率,得到半抑制浓度IC_(50)。双倒数作图法判断黄芪多糖对黄嘌呤氧化酶的抑制类型,并计算抑制常数K_i值,等毒法评价黄芪多糖与别嘌呤醇的联合作用效果。黄芪多糖能够抑制黄嘌呤氧化酶的活性,其IC_(50)为1.37 mg/mL。动力学分析表明,黄芪多糖对黄嘌呤氧化酶为竞争性可逆抑制作用,抑制常数K_(i)为0.59 mg/mL。黄芪多糖与别嘌呤醇的联合作用表现为拮抗作用,黄芪多糖对黄嘌呤氧化酶的活性有抑制作用。
Xanthine oxidase is a key enzyme in uric acid formation,which can effectively reduce the content of uric acid in vivo by inhibiting the activity of xanthine oxidase.To explore the inhibitory effects of Astragalus polysaccharide on xanthine oxidase,the variation of uric acid content of reaction system per unit time was determined by UV-Spectrophotometry,and the inhibition ratio of Astragalus polysaccharide to xanthine oxidase was calculated.The semi-inhibitory concentration IC_(50) was also obtained.The inhibition type of Astragalus polysaccharide on xanthine oxidase was determined by the Lineweaver-Burk plot,and a Dixon plot was used to obtain the inhibition kinetic parameter(Ki).The inhibitory effects of Astragalus polysaccharide combining allopurinol were explored by the toxicological studies.Astragalus polysaccharide could inhibit the activity of xanthine oxidase,and the IC_(50) was 1.37 mg/m L.The kinetic analysis showed that Astragalus polysaccharide had competitive and reversible inhibitory effect on xanthine oxidase,with an inhibition constant(K_(i))of 0.59 mg/m L.There was antagonism effect when Astragalus polysaccharide was combined with allopurinol.Astragalus polysaccharide could inhibit the activity of xanthine oxidase.
作者
李晶
姜丽丽
李沐轩
邓凤
徐宋瑶
薛昕
扈瑞平
薛慧婷
LI Jing;JIANG Lili;LI Muxuan;DENG Feng;XU Songyao;XUE Xin;HU Ruiping;XUE Huiting(College of Basic Medical,Inner Mongolia Medical University,Hohhat 010110,China;Affiliated Hospitalof Inner Mongolia Medical University,Hohhat 010110,China)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2021年第10期16-20,共5页
Journal of Food Science and Biotechnology
基金
国家自然科学基金项目(32050217)
内蒙古自治区自然科学基金项目(2019BS08013)
内蒙古自治区高等学校科学研究项目(NJZY19100)
内蒙古蒙医药协同创新项目项目(MYYXT201901)
内蒙古自治区科技计划项目(2019GG160)
内蒙古医科大学人才团队项目(NYTD-2018003)
内蒙古医科大学第一临床医学院2020年大学生“启蒙计划”项目(FYQMJH2020037)。
关键词
黄芪多糖
黄嘌呤氧化酶
抑制作用
动力学分析
Astragalus polysaccharide
xanthine oxidase
inhibitory effect
kinetic analysis
作者简介
通信作者:姜丽丽(1978-),女,硕士,高级实验师,主要从事药用植物分子生物学方面的研究。E-mail:jianglili1978@126.com。
