摘要
The devastating coronavirus disease 2019(COVID-19)pandemic has prompted worldwide efforts to study structural biological traits of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and its viral components.Compared to the Spike protein,which is the primary target for currently available vaccines or antibodies,knowledge about other virion structural components is incomplete.Using high-resolution mass spectrometry,we report a comprehensive post-translational modification(PTM)analysis of nucleocapsid phosphoprotein(NCP),the most abundant structural component of the SARS-CoV-2 virion.In addition to phosphoryl groups,we show that the SARS-CoV-2 NCP is decorated with a variety of PTMs,including N-glycans and ubiquitin.Based on newly identified PTMs,refined protein structural models of SARS-CoV-2 NCP were proposed and potential immune recognition epitopes of NCP were aligned with PTMs.These data can facilitate the design of novel vaccines or therapeutics targeting NCP,as valuable alternatives to the current vaccination and treatment paradigm that is under threat of the ever-mutating SARS-CoV-2 Spike protein.
基金
supported by the National Natural Science Foundation of China(U20A20343)
the National Key Research and Development Program(2017YFC1200204)
Emergency Project of Zhejiang Provincial Department of Science and Technology(2020C03123-1)
Fundamental Research Funds for the Central Universities(2018XZZX001-13).
作者简介
contributed equally to this work:Zeyu Sun;contributed equally to this work:Xiaoqin Zheng;Corresponding author:Lanjuan Li,State Key Laboratory for the Diagnosis and Treatment of Infectious Diseases,The First Affiliated Hospital,Zhejiang University School of Medicine,No.79 Qingchun Road,Shangcheng District,Hangzhou 310003,China.E-mail:ljli@zju.edu.cn。
