摘要
为探讨麦麸阿魏酰低聚糖(Feruloyl oligosaccharides,FOs)对大鼠肝脏组织抗氧化功能的影响,选取40只健康断奶大鼠,随机分为5组,包括空白对照组(生理盐水)、阳性对照组(w(Vc)=100 mg/kg)、低剂量组(w(FOs)=20mg/kg)、中剂量组(w(FOs)=40mg/kg)和高剂量组(w(FOs)=80mg/kg)。试验期为21d,每日定时灌胃。试验结束后,收集肝脏组织,通过酶联免疫吸附试验法测定其抗氧化相关指标,利用RT-PCR和Western Blot技术分别分析抗氧化相关基因的mRNA和蛋白表达量。结果表明:1)随着FOs剂量增加,谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)和超氧化物岐化酶(Superoxide dismutase,SOD)活性,谷氨酸-半胱氨酸连接酶催化亚基(Glutamate cysteine ligase catalytie subunit,GCLC)、醌氧化还原酶1(NAD(P)Hquinoneoxidoreductasel,NQO1)、CAT、SOD、GSH-Px和核因子E2相关因子2(Nuclear factor erythroid 2p45-related factor 2,Nrf2)的mRNA表达水平二次方增加,Nrf2蛋白表达水平线性增加(P<0.05),而8-羟基脱氧鸟苷(8-hydroxy deoxyguanosine,8-OHdG)含量线性和二次方极显著下降(P<0.01)。2)与Vc组相比,低剂量组中的GSH-Px活性和8-OHdG含量极显著降低(P<0.01),而CAT的mRNA表达水平显著增加(P<0.05),中剂量组中的总抗氧化能力(Total antioxidant capacity,T-AOC)、过氧化氢酶(Catalase,CAT)活性和8-OHdG含量显著降低(P<0.05),而GCLC、NQO1、CAT、SOD、GSH-Px和Nrf2的mRNA表达水平显著增加(P<0.05),高剂量组中的SOD和GSH-Px活性和8-OHdG含量显著降低(P<0.05)。综上,FOs可增强肝脏抗氧化酶活性,减少DNA氧化损伤,且可提高Nrf2及其下游抗氧化基因的mRNA表达水平,且以中剂量40mg/kg BW的使用剂量效果最佳。
To investigate the effects of wheat bran feruloylated oligosaccharides(FOs)on the antioxidant capacity in rat liver,forty healthy weaned rats were randomly assigned to five groups,including control(normal saline),positive control(w(Vc)=100 mg/kg),low-dose(w(FOs)=20 mg/kg),medium-dose(w(Fos)=40 mg/kg),and high-dose(w(FOs)=80 mg/kg)FOs groups.The experiment duration was 21 d.The normal saline,Vc and FOs were given by gavage every day.At the end of the experiment,the liver tissues were collected to measure antioxidant related indexes by ELISA.The mRNA expression of antioxidant related genes and the protein expression of Nrf2 were determined using RT-PCR and Western Blot,respectively.The results showed that:1)With the increase of FOs dosage,the SOD and GSH-Px activities,GCLC,NQO1,CAT,SOD,GSH-Px and Nrf2 mRNA expression level were increased quadratically,the protein expression level of Nrf2 was increased linearly(P<0.05),whereas 8-OHdG concentrations was decreased linearly and quadratically(P<0.01).2)Compared with Vc,low-dose group had greater CAT mRNA expression level(P<0.05),and lower GSH-Px activity and 8-OHdG concentrations(P<0.01);medium-dose group had greater GCLC,NQO1,CAT,SOD,GSH-Px and Nrf2 mRNA expression level(P<0.05),and lower T-AOC,CAT activity and 8-OHdG concentrations(P<0.05));high-dose group had lower GSH-Px activity and 8-OHdG concentrations(P<0.05).In conclusion,FOs could increase antioxidant enzymes activity,decrease DNA oxidative damage,and increase mRNA expression level of Nrf2 and downstream antioxidant genes.The suitable dietary supplemental level of FOs is 40 mg/kg BW.
作者
王文文
段元霄
王园
孟子琪
安晓萍
齐景伟
WANG Wenwen;DUAN Yuanxiao;WANG Yuan;MENG Ziqi;AN Xiaoping;QI Jingwei(College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China;Inner Mongolia Herbivorous Livestock Feed Engineering and Technology Research Center,Hohhot 010018,China;Institute of Animal Nutrition and feed Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2021年第9期137-145,共9页
Journal of China Agricultural University
基金
内蒙古自然科学基金项目(2020MS03041)
内蒙古自治区关键技术攻关计划项目(2020GG0030)
内蒙古农业大学科技成果转化专项资金(YZGC2017025)
内蒙古自治区科技重大专项(2020ZD0004)。
关键词
阿魏酰低聚糖
大鼠
肝脏
抗氧化
feruloylated oligosaccharide(FOs)
rat
liver
antioxidant capacity
作者简介
第一作者:王文文,博士研究生,E-mail:wangwenwen2017@emails.imau.edu.cn;通讯作者:王园,副教授,主要从事生物饲料的研发与应用研究,E-mail:wangyuan.926@163.com。
