摘要
花叶矢竹是一种珍稀观赏竹,以花叶矢竹愈伤组织脱分化的无根试管苗为试验材料,研究NAA、IBA、6-BA、TDZ以及激素组合对花叶矢竹不定根诱导和壮苗生长的影响。结果表明:NAA对花叶矢竹不定根诱导有主导作用,效果优于单独添加IBA、远优于组合添加NAA、IBA、6-BA。NAA浓度0.5~2.0 mg·L^(-1)时50 d生根率均可达100%,其中浓度0.5 mg·L^(-1)芽苗生根最快,长势最好,15 d生根率已达80%,30 d生根率达100%,平均生根数6.95条,平均根长0.995 cm。NAA浓度0.2~0.5 mg·L^(-1)时,壮苗效果好,且增殖系数可达8.5,平均新芽长可达1.595 cm。添加活性炭、6-BA、TDZ对生根和竹苗生长有抑制作用。
The effects of NAA,IBA,6-BA and TDZ on adventitious root induction and seedling strength of Pseudosasa japonica f.Akebonosuji were studied by using tissue culture seedlings as experimental materials.The results showed that NAA played a leading role in adventitious root induction,and the effect was better than that of IBA alone,much better than that of the combination of NAA,IBA and 6-BA.When the concentration of NAA was 0.5-2.0 mg·L^(-1),the rooting rate reached 100%in 50 days.Under the concentration of 0.5 mg·L^(-1),the rooting rate was 80%in 15 days and 100%in 30 days.The average rooting number was 6.95 and the average root length was 0.995cm.When NAA concentration was 0.2-0.5 mg·L^(-1),the seedling effect was good,the multiplication coefficient was up to 8.5,and the average new bud length amounted to 1.595cm.The addition of activated carbon,6-BA or TDZ could inhibit rooting and proliferation.
作者
高会彬
郑仁红
周国强
杨海芸
余英
GAO Hui-bin;ZHENG Ren-hong;ZHOU Guo-qiang;YANG Hai-Yun;YU Ying(Forestry and Bamboo Industry Research Institute of Yibin,Yibin 644000,Sichuan,China;The Nurturing Station of the State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)
出处
《竹子学报》
2021年第1期44-51,共8页
Journal of Bamboo Research
基金
宜宾市引进高层次人才项目(2018YG02)
国家自然科学基金(31901370)。
关键词
花叶矢竹
组培苗
生根
壮苗
Pseudosasa japonica f.akebonosuji
Tissue culture seedling
Rooting
Strengthening Seedlings
作者简介
高会彬,硕士研究生,从事植物组培研究。E-mail:1007420254@qq.com;通信作者:余英,高级工程师,从事植物组培研究。E-mail:1730688@qq.com。
