摘要
为探究冷鲜猪肉在贮藏过程中细菌群落结构的演替规律。将新鲜猪肉使用非密封包装进行低温贮藏,每天取样1次(共10 d),测定每日样品的pH值和挥发性盐基氮含量,并通过16S rDNA高通量测序技术分析细菌的多样性、群落组成和演替规律,探究细菌群落变化与腐败指标之间的相关性。通过Alpha多样性分析可知,猪肉在冷藏过程中细菌丰度和多样性总体上呈“三级台阶”的下降,丰度和多样性最高点在D3。在门水平上优势菌为变形菌门、厚壁菌门和拟杆菌门;在目水平上优势菌为假单胞菌目和肠杆菌目;在属水平上优势菌为假单胞菌属、不动杆菌属和泛菌属。对各菌属相对丰度变化的分析表明假单胞菌属,与肠杆菌属、泛菌属之间存在互生关系。细菌相对丰度和腐败指标的相关性表明猪肉腐败过程中拉乌尔菌属和醋杆菌属的相对丰度与挥发性盐基氮含量具有较强的相关性。
To explore the dominant flora of chilled fresh pork in the process of spoilage and to understand the succession law of bacterial community.Fresh pork was refrigerated in a non-sealed package until completely spoiled,sampled once a day(total 10 days),to determined the pH and TVB-N content of the daily samples,and then sequenced all of samples by using 16s rDNA,analysis of bacterial diversity,community composition and succession law.And explore the correlation between bacterial abundance and corruption indicators.According to the analysis of Alpha diversity,the bacterial abundance and diversity of pork in the refrigeration process generally decreased in the form of'three steps',and the highest abundance and diversity was at D3.At the level of phylum,the dominant bacteria are Proteobacteria,Firmicutes and Bacteroidetes.At the order level,the dominant bacteria were Pseudomonadales and Enterobacteriales.At the genus level,the dominant bacteria were Pseudomonas spp.,Acinetobacter spp,and Pantoea spp.Through the analysis of the relative abundance change of each genus,it was found that Pseudomonas spp.had an alternate relationship with Enterobacter spp.and Serratia spp.Through the correlation analysis of the relative abundance of bacteria and the content of putrid substances,it was found that the relative abundance of Raoultella spp.and Citrobacter spp was strongly correlated with the content of TVB-N.
作者
王新惠
孙劲松
赵芮
潘攀
丁悦
刘力嘉
刘洋
Wang Xinhui;Sun Jinsong;Zhao Rui;Pan Pan;Ding Yue;Liu Lijia;Liu Yang(Chengdu University,Key Laboratory of Meat Processing of Sichuan,Chengdu 610106)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2021年第9期233-240,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金面上项目(31772093)
四川省科技厅应用项目(2018JY0365)
大学生省级创新计划项目(S201911079099)。
关键词
冷鲜猪肉
高通量测序
优势菌
群落结构
chilled pork
high-throughput sequencing
dominant bacteria
community structure
作者简介
王新惠(1982-),女,博士,教授;通信作者:刘洋,E-mail:shuimu119@126.com。
