摘要
目的分析基质金属蛋白酶(MMP)-2、血管内皮生长因子(VEGF)在食管鳞癌中的表达及其与预后的关系。方法收集2015年1月至2016年2月在郑州市第九人民医院接受治疗的146例食管鳞癌患者的癌组织及同期在郑州市第九人民医院体检正常的60例健康者的食管组织作为研究对象。使用免疫组化法检测组织中MMP-2、VEGF的表达水平,并以COX多因素回归分析及生存曲线分析其与患者临床病理特征及预后的关系。结果食管鳞癌患者组织中MMP-2阳性率(53.42%)高于正常组织(8.33%)(P<0.05);VEGF阳性率(78.77%)高于正常组织(18.33%)(P<0.05)。相关性检验显示MMP-2、VEGF在食管鳞癌组织中的表达具有相关性(χ^(2)=7.086,P<0.05);MMP-2、VEGF的阳性表达与食管鳞癌患者TNM分期、分化程度、浸润程度及淋巴结是否转移有关(P<0.05),与性别、年龄、肿瘤大小无关(P<0.05)。对146例食管鳞癌患者进行为期5 a的随访,结果显示MMP-2阳性组生存率[19.23%(15/78)]低于阴性组[61.54%(48/78)],差异有统计学意义(P<0.05);VEGF阳性组生存率[33.04%(38/115)]低于阴性组[80.65%(25/31)],差异有统计学意义(P<0.05)。COX多因素回归分析结果显示,MMP-2、VEGF阳性表达为影响食管鳞癌患者生存期的独立危险因素(P<0.05)。结论食管鳞癌组织中MMP-2、VEGF均呈阳性表达,且具有相关性,其表达水平与TNM分期、分化程度、浸润程度及淋巴结是否转移有关,为影响食管鳞癌患者预后的独立危险因素,有望成为预测食管鳞癌患者预后的有效指标。
Objective To analyze the expression of matrix metalloproteinase(MMP)-2 and vascular endothelial growth factor(VEGF)in esophageal squamous cell carcinoma and their relationship with prognosis.Methods The cancer tissues of 146 patients with esophageal squamous cell carcinoma who were treated in Ninth People’s Hospital of Zhengzhou from January 2015 to February 2016 were collected,and the normal esophageal tissues of 60 healthy people with normal physical examination in Ninth People’s Hospital of Zhengzhou during the same period were collected,and the levels of MMP-2 and VEGF of the tissues were detected by immunohistochemical method.COX multivariate regression analysis and survival curve were used to analyze their relationship with clinicopathological characteristics and prognosis.Results The positive rate of MMP-2 of patients with esophageal squamous cell carcinoma(53.42%)was significantly higher than that of the normal tissues(8.33%)(P<0.05),and the positive rate of VEGF(78.77%)was significantly higher than that of the normal tissues(18.33%)(P<0.05).Correlation test showed that the expression of MMP-2 and VEGF was correlated with esophageal squamous cell carcinoma(χ^(2)=7.086,P<0.05).The positive expression of MMP-2 and VEGF was related to the TNM staging,degree of differentiation,degree of invasion and lymph node metastasis in patients with esophageal squamous cell carcinoma(P<0.05).A 5-year follow-up of 146 patients with esophageal squamous cell carcinoma showed that the survival rate of MMP-2 positive group was[19.23%(15/78)]lower than that of the negative group[61.54%(48/78)],the difference was statistically significant(P<0.05).The survival rate of VEGF positive group[33.04%(38/115)]was lower than that of the negative group[80.65%(25/31)],and the difference was statistically significant(P<0.05).COX multivariate regression analysis showed that the positive expression of MMP-2 and VEGF were independent risk factors affecting the survival of patients with esophageal squamous cell carcinoma(P<0.05).Conclusion Both MMP-2 and VEGF are positively expressed in esophageal squamous cell carcinoma tissues,and they are correlated.The expression levels are related to TNM stage,degree of differentiation,degree of invasion and lymph node metastasis,and all the above are independent risk factors affecting the prognosis of patients with esophageal squamous cell carcinoma.It is expected to become an effective indicator for predicting the prognosis of patients with esophageal squamous cell carcinoma.
作者
张文宇
ZHANG Wenyu(Department of Cardiothoracic Surgery,Ninth People’s Hospital of Zhengzhou,Zhengzhou 450000,China)
出处
《河南医学研究》
CAS
2021年第25期4621-4625,共5页
Henan Medical Research
关键词
食管鳞癌
血管内皮生长因子
基质金属蛋白酶
预后
esophageal squamous cell carcinoma
vascular endothelial growth factor
matrix metalloproteinases
prognosis
作者简介
通信作者:张文宇,E-mail:wyzhang0983@163.com。
