摘要
目的:探索用细胞块制备试剂盒对眼内玻璃体液微量细胞制备细胞块的成功率,苏木素-伊红(hematoxylin-eosin,HE)染色效果及技术要点。方法:收集中山大学中山眼科中心临床病理科2020年9月至2021年1月由临床送检的25例玻璃体液(含玻璃体切割液),应用细胞块制备试剂盒制备细胞块后,常规固定、脱水、包埋、切片,随后进行HE染色,观察染色效果。结果:25例玻璃体细胞蜡块制作成功率达到100%,制片后HE染色效果好,背景干净,细胞形态清晰,核质对比分明。结论:应用细胞块制备试剂盒能将眼内玻璃体液微量细胞制成蜡块,极大提高了标本的利用率,为后续的病理研究提供丰富的材料。
Objective:To explore the effect and technical key points of the cell block preparation kit for collecting a few cells in ocular vitreous humor.Methods:A total of 25 cases of vitreous humor(including vitrectomy fluid)were collected from Zhongshan Ophthalmic Center,Sun Yat-sen University from September 2020 to January 2021.Cell block preparation kit was used to prepare cell blocks,which were routinely fixed,dehydrated,embedded,sectioned,and then hematoxylin-eosin(HE)stained.Results:The success rate of 25 cases of vitreous cell paraffin blocks reached 100%,and the morphology of the cells was clear with clean background and shape contrast of nucleus and plasma in HE staining.Conclusion:The cell block preparation kit can make the cells of intraocular vitreous humor into paraffin blocks,which greatly improves the utilization rate of specimens and is conducive to providing abundant materials for pathological studies.
作者
陈淑霞
林健贤
高欢欢
唐丽娟
张文忻
李永平
张平
CHEN Shuxia;LIN Jianxian;GAO Huanhuan;TANG Lijuan;ZHANG Wenxin;LI Yongping;ZHANG Ping(State Key Laboratory of Ophthalmology,Zhongshan Ophthalmic Center,Sun Yat-sen University,Guangzhou 510060,China)
出处
《眼科学报》
CAS
2021年第8期585-589,共5页
Eye Science
作者简介
通信作者:张平,Email:zhangping@gzzoc.com。
