摘要
本试验旨在探讨D-天冬氨酸(D-Asp)对葡聚糖硫酸钠(DSS)诱导的小鼠结肠炎肠道损伤的恢复效果。试验选取40只体重相近的C57BL/6J雄性小鼠,随机分为5组,即对照组、0 mmol/L D-Asp组、0.15 mmol/L D-Asp组、2.30 mmol/L D-Asp组和7.70 mmol/L D-Asp组,每组8个重复,每个重复1只,单笼饲养。试验第1~7天,对照组小鼠正常饮用蒸馏水,其他各组小鼠均饮用含3.5%DSS的蒸馏水,诱导小鼠结肠炎。试验第8天开始,对照组和0 mmol/L D-Asp组小鼠直肠灌注0.1 mL生理盐水,其他各组分别直肠灌注0.1 mL浓度为0.15、2.30和7.70 mmol/L的D-Asp溶液,每隔1 d灌注1次,共灌注5次。结果表明:1)与对照组相比,0 mmol/L D-Asp组小鼠第5天体重和平均日采食量显著降低(P<0.05),结肠长度变短并出现浸血现象。与0 mmol/L D-Asp组相比,7.70 mmol/L D-Asp组小鼠第12~14天体重和平均日采食量显著提高(P<0.05),结肠变长,绒毛高度增高及隐窝深度变浅,杯状细胞排列更规则。2)与0 mmol/L D-Asp组相比,7.70 mmol/L D-Asp组的血清白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)含量和嗜酸性粒细胞过氧化物酶(EPO)活性显著降低(P<0.05),结肠中天冬氨酸/谷氨酸载体2(SLC25A13)mRNA相对表达量显著升高(P<0.05),结肠中白细胞介素-6(IL-6)、乙酰辅酶A羧化酶(ACC)和天冬氨酸/谷氨酸载体1(SLC25A12)mRNA相对表达量显著降低(P<0.05)。由此可见,直肠灌注D-Asp可通过提高抗氧化酶的活性和相关基因的表达,抑制炎性细胞因子的分泌,改善结肠形态结构,从而促进因DSS引起的小鼠结肠炎损伤修复。
This experiment was conducted to study the repairing effects of D-aspartic acid(D-Asp)on dextran sodium sulfate(DSS)induced colonic intestinal injury of mice.A total of 40 C57BL/6J male mice with similar body weight were randomly divided into 5 groups,which were the control group,0 mmol/L D-Asp group,0.15 mmol/L D-Asp group,2.30 mmol/L D-Asp group and 7.70 mmol/L D-Asp group,each group contained 8 replicates and each replicate contained 1 mouse,the mice were reared in a single cage.During days 1 to 7,the mice in the control group drank sterile water,while the others in other groups drank sterile water containing 3.5%DSS,to induce colitis of mice.From day 8,the control group and 0 mmol/L D-Asp group were rectal perfused with 0.1 mL normal saline,and the mice in the other groups were rectal perfused with 0.1 mL D-Asp at the concentrations of 0.15,2.30 and 7.70 mmol/L,respectively,and perfused once every 1 d for 5 times.The results showed as follows:1)compared with the control group,the body weight on day 5 and average daily feed intake of 0 mmol/L D-Asp group were significantly decreased(P<0.05),and the colon length was shortened and the phenomenon of blood infiltration occurred.Compared with the 0 mmol/L D-Asp group,the body weight on days 12 to 14 and average daily feed intake of 7.70 mmol/L D-Asp group were significantly increased(P<0.05),the colon became longer,the villi height and crypt depth became shallower,and the arrangement of goblet cells was more regular.2)Compared with the 0 mmol/L D-Asp group,the serum interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)contents and eosinophil peroxidase(EPO)activity of 7.70 mmol/L D-Asp group were significantly decreased(P<0.05),the mRNA relative expression level of aspartate/glutamate carrier 2(SLC25A13)in colon was significantly increased(P<0.05),and the mRNA relative expression levels of interleukin-6(IL-6),acetyl CoA carboxylase(ACC)and aspartate/glutamate carrier 1(SLC25A12)in colon were significantly decreased(P<0.05).In conclusion,rectal perfused D-Asp can inhibit the secretion of inflammatory cytokines and improve the morphological structure of colon by increasing the activity of antioxidant enzymes and the expression of related genes,thereby promoting the repair of mouse colitis damage caused by DSS.
作者
胡鲜
王晨昱
刘娣
龙静
范文君
何流琴
李铁军
姚继明
HU Xian;WANG Chenyu;LIU Di;LONG Jing;FAN Wenjun;HE Liuqin;LI Tiejun;YAO Jiming(Hunan Province Key Laboratory of Animal Intestinal Function and Regulation,College of Life Sciences,Hunan Normal University,Changsha 410081,China;Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Processes,National Engineering Laboratory of Poultry Breeding Pollution Control and Resource Technology,Institute of Subtropical Agricultural Ecology,Chinese Academy of Sciences,Changsha 410125,China;University of Chinese Academy of Sciences,Beijing 100049,China;Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;Guangdong Wangda Group Co.,Ltd.,Guangzhou 510663,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2021年第8期4708-4718,共11页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金项目(31902168,31872371)
中国科协青年托举人才项目(2019QNRC001)
广西桂林技术创新引导计划(2020010901)
广西重点研发计划(桂科AB19259012)
长沙市科技计划项目(kq1907073)。
作者简介
胡鲜(1996-),女,贵州普安人,硕士研究生,从事动物营养研究。E-mail:1654027745@qq.com;通信作者:何流琴,教授,博士生导师,E-mail:heliuqin@hunnu.edu.cn;通信作者:姚继明,畜牧师,E-mail:wangdayaojiming@163.com。
