摘要
为解决(S)-奥美拉唑制备过程中存在的催化剂活性低的问题,采用微乳液体系,利用豆荚过氧化物酶(soybean pod peroxidase,SPP)在十六烷基三甲基溴化铵(hexadecyl trimethyl ammonium bromide,CTAB)/氯仿/正丁醇/水油包水微乳液中催化奥美拉唑硫醚制备(S)-奥美拉唑.利用响应面法(response surface methodology,RSM)优化反应条件,建立了响应面模型,获得最优反应条件,使得(S)-奥美拉唑的转化率、收率和对映体过量百分率(enantiomeric excess,e.e.)分别达到93.75%、91.56%、96.08%.实验同时证明SPP成本低、对映体选择性好、热稳定性好,可替代成本较高的辣根过氧化物酶(horseradish peroxidase,HRP)用于生物催化反应.
In order to solve the problem of low catalyst activity in the preparation of(S)-omeprazole,a microemulsion system was used to prepare(S)-omeprazole from(S)-omeprazole thioether catalyzed by soybean pod peroxidase(SPP)in hexadecyl trimethyl ammonium bromide(CTAB)/chloroform/n-butanol/water-in-oil microemulsion.The response surface methodology(RSM)was used to optimize the reaction conditions,and the response surface model was established to obtain the optimal reaction conditions.The conversion rate,yield and enantiomeric excess percentage of(S)-omeprazole reached 93.75%,91.56%and 96.08%,respectively.The experiment also demonstrated that SPP has low cost,good enantioselectivity,and good thermal stability,and it’s able to replace the higher cost horseradish peroxidase(HRP)for biocatalytic reactions.
作者
王志勇
李德鹏
李惠玲
王繁业
WANG Zhiyong;LI Depeng;LI Huiling;WANG Fanye(School of Chemical Engineering,Qingdao University of Science and Technology,Qingdao,Shandong 266042,China)
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2021年第4期597-602,共6页
Journal of Central China Normal University：Natural Sciences
基金
山东省重点研发计划项目(2019GSF107027,2019GNC106028,2019GSF107033)。
关键词
油包水微乳液
奥美拉唑
豆荚过氧化物酶
响应面法
手性亚砜
water-in-oil microemulsion
omeprazole
soybean pod peroxidase
response surface optimization
chiral sulfoxides
作者简介
通信联系人:王繁业,E-mail:lwz_618@163.com。
