摘要
目的探讨富含半胱氨酸的酸性分泌糖蛋白(Sparc蛋白)对人瘢痕疙瘩成纤维细胞(HKF)、正常人成纤维细胞(NFs)增殖与凋亡以及对于其细胞外基质中Ⅰ胶原与Ⅲ型胶原表达的影响。方法分离培养HKF以及NFs,将2种细胞分为实验组与对照组,实验组加入Sparc蛋白孵育,对照组加入等量培养基进行处理。CCK-8细胞增殖实验检测Sparc蛋白对各组细胞增殖的影响;流式细胞实验检测Sparc蛋白对各组细胞凋亡的影响;Western blot与RT-PCR检测各组细胞Ⅰ型胶原与Ⅲ型胶原的表达。结果Sparc蛋白可以显著促进各组细胞的增殖,抑制其凋亡,Sparc蛋白可以显著促进各组细胞Ⅰ型胶原与Ⅲ型胶原的表达。结论Sparc蛋白是促进瘢痕疙瘩形成的重要蛋白,可靶向调控抑制瘢痕疙瘩的生成。
Objective To investigate the effect of secreted protein,acidic and rich in cysteine(Sparc)on the proliferation and apoptosis of human keloid fibroblasts(HKF)and normal fibroblasts(NFs),as well as the expression of collagen typeⅠand collagen typeⅢin the extracellular matrix.Methods HKF and NFs were isolated and cultured.The two types of cells were both divided into experimental group and control group in which the experimental group was incubated with Sparc while the control group was incubated with the same amount of DMEM.CCK-8 experiment was used to test the influence of Sparc on each cell proliferation;flow cytometry was used to test the influence of Sparc on each cell apoptosis;Western blot and RT-PCR were used to detect the expression of collagen typeⅠand collagen typeⅢon each group.Results SPARC could significantly promote the proliferation and inhibit the apoptosis of cells in each group,and SPARC could obviously promote the expression of collagen type I and Collagen typeⅢin each group.Conclusion SPARC is an important protein to promote the formation of keloids,which can be targeted to regulate the formation of keloids.
作者
朱晓璇
李心怡
王佳妮
丁以春
李小静
Zhu Xiaoxuan;Li Xinyi;Wang Jiani(Dept of Plastic Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei 230022;Dept of Plastic Surgery, The First People’s Hospital of Hefei, Hefei 230031)
出处
《安徽医科大学学报》
CAS
北大核心
2021年第8期1222-1225,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省自然科学基金(编号:9021548201)。
作者简介
朱晓璇,女,硕士研究生;责任作者:李小静,女,教授,E-mail:lixiaojing5@163.com。
