摘要
【目的】研究不同鲜食玉米种质资源对南方锈病的抗性表现,为抗病品种选育提供材料及理论依据。【方法】结合田间自然感病与人工接种感病目测鉴定调查,开展自交系对南方锈病的抗性分析。【结果】不同鲜食玉米自交系对南方锈病的抗性存在显著差异,在710份材料田间自然感病鉴定中,1.11%表现高抗水平,8.34%表现为抗病水平;中抗和感病水平的材料最多,占77.75%,其余材料表现为高感水平。结合人工接种鉴定,筛选得到5份高抗南方锈病的自交系,包括3份糯玉米材料、1份甜玉米材料和1份甜加糯玉米材料。对抗病自交系09N1-1-1初步遗传分析显示,其后代F2和BC1F1群体抗感分离结果符合3∶1(χ^(2)值2.473,P=0.116)和1∶1(χ^(2)值0.084,P=0.772)分离比例,表明自交系09N1-1-1对南方锈病的抗性可能由显性单基因控制。【结论】研究表明,现有鲜食玉米存在高抗南方锈病的资源,筛选利用抗病种质对当前品种选育具有潜在的应用价值。
【Objective】The resistance of fresh corn germplasm resources to southern corn rust was explored with a view to provide materials and theoretical references for resistant varieties.【Method】Investigation was conducted to analyze the resistance of different inbred lines to southern corn rust with the method of visual observation under field natural conditions and artificial inoculation.【Result】There were significant differences between different inbred lines of fresh corn.Under field natural conditions,only 1.11%of 710 tested inbred lines showed highly resistant level,and 8.34%of which showed resistant level.About 77.75%of inbred lines showed middle resistant and susceptible levels,while the rest showed highly susceptible level.After artificial inoculation,5 inbred lines of fresh corn that highly resistant to the southern corn rust were screened out,including three of waxy corn,one of sweet corn and one of waxy-sweet corn.Preliminary genetic analysis of the resistant inbred line 09N1-1-1 showed that the separation ratios of F2 and BC1F1 populations were 3∶1(χ^(2)=2.473,P=0.116)and 1∶1(χ^(2)=0.084,P=0.772)respectively,revealing the resistance of inbred line 09N1-1-1 was probably controlled by a single dominant gene.【Conclusion】The study demonstrated that the current fresh corn resources have high resistance to southern corn rust.Therefore,the selection and utilization of disease resistant germplasm has potential application in variety breeding currently.
作者
田耀加
王秋燕
吴蓓
叶伟忠
陈红弟
TIAN Yaojia;WANG Qiuyan;WU Bei;YE Weizhong;CHEN Hongdi(Guangzhou Academy of Agricultural Sciences,Guangzhou 510308,China)
出处
《广东农业科学》
CAS
2021年第7期111-117,共7页
Guangdong Agricultural Sciences
基金
广州市珠江科技新星(201806010108)
广州市2021年农业财政专项资金(20101264)。
关键词
鲜食玉米
南方锈病
种质资源
抗性鉴定
遗传分析
fresh corn
southern corn rust
germplasm resource
resistance identification
genetic analysis
作者简介
田耀加(1983-),男,博士,高级农艺师,研究方向为玉米遗传育种,E-mail:2602333562@qq.com。
