摘要
为挖掘控制大麦籽粒苯丙基酸含量的QTL,以紫光芒裸二棱和Schooner构建的包含193个家系的重组自交系(RIL)为材料,测定RIL群体及亲本籽粒苯丙氨酸含量,并结合SSR标记和完备区间作图法构建遗传连锁图谱,对大麦籽粒苯丙氨酸含量进行QTL定位。结果表明,紫光芒裸二棱籽粒苯丙氨酸含量为1.23mg·g^(-1),Schooner籽粒苯丙氨酸含量为0.60 mg·g^(-1),群体籽粒苯丙氨酸含量在0.59~1.24mg·g^(-1)之间;所构建的大麦遗传连锁图谱包含180对SSR标记,总遗传距离为2671.03 cM,平均标记间距为14.84 cM;共检测到4个控制大麦籽粒苯丙氨酸含量的QTL,均为新发现的QTL,除qPHE-4H加性效应来自母本紫光芒裸二棱外,其他3个QTL加性效应均来自父本Schooner。qPHE-2H和qPHE-7H为主效QTL,分别位于2H和7H染色体上,表型贡献率分别为12.32%和15.45%。该研究结果为大麦籽粒苯丙氨酸含量QTL精细定位奠定了基础。
In order to explore QTL of phenylalanine content in grains of barley,a total of 193 recombinant inbred lines derived from the cross between Ziguangmangluoerleng(ZGMLEL)and Schooner were used as materials.Grain phenylalanine content in grains of the populations and their parents were determined.The genetic linkage map was constructed by SSR markers and complete interval mapping method.QTLs for phenylalanine content in grains were mapped.Results showed that phenylalaninecontentin grains were 1.23 and 0.60 mg·g^(-1) for ZGMLEL and Schooner,respectively.The content of phenylalanine in recombinant inbred lines ranged from 0.59 mg·g^(-1) to 1.24 mg·g^(-1).The genetic linkage map contains 180 pairs of SSR markers with the total genetic distance of 2671.03 cM,and the average interval of 14.84 cM.4 QTLs for phenylalanine content in grains of barley were mapped,all of which were newly discovered.Except for the additive effect of qPHE-4 H from the female parent ZGMLEL,the other 3 QTLs were from the male parent Schooner.qPHE-2 H and qPHE-7 H were major QTLs located on chromosomes 2 H and 7 H,respectively;and their phenotype contribution rates were 12.32%and 15.45%,respectively.This study laid foundation for fine mapping QTL of phenylalanine content in barley grains.
作者
严苓方
杨晓梦
杜娟
孙正海
普晓英
杨加珍
曾亚文
YAN Lingfang;YANG Xiaomeng;DU Juan;SUN Zhenghai;PU Xiaoying;YANG Jiazhen;ZENG Yawen(South and Southeast Asia Joint R&D Center of Economic Forest Full Industry Chain of Yunnan Province/International Technologial Cooperation Base of High Effective Economic Forestry Cultivating of Yunnan Province/College of Landscape and Horticulture,Southwest Forestry University,Kunming,Yunnan 650224,China;Biotechnology and Germplasm Resources Institute,Yunnan Academy of Agricultural Sciences/Agricultural Biotechnology Key Laboratory of Yunnan Province,Kunming,Yunnan 650205,China;Key Laboratory of the Southwestern Crop Gene Resources and Germplasm Innovation,Ministry of Agriculture,Kunming,Yunnan 650205,China)
出处
《麦类作物学报》
CAS
CSCD
北大核心
2021年第7期851-856,共6页
Journal of Triticeae Crops
基金
国家现代农业产业技术体系建设专项(CARS-05-01A-04)
国家自然科学基金项目(31260326)
云南省重点研发计划项目(2019IB011,2018BB013,2016RA032)。
作者简介
第一作者:严苓方,E-mail:yanlingfang1997@163.com;杨晓梦,yxm89ccf@126.com,与第一作者同等贡献;杜娟,cookoo1976@126.com,与第一作者同等贡献;通讯作者:孙正海(E-mail:sunzhenghai1978@163.com);通讯作者:曾亚文(E-mail:zengyw1967@126.com)。
