摘要
以七味白术散总苷的提取率为响应值,煎煮时间、提取次数、料液比为考察因素,采用Box-Behnken响应面法设计3因素3水平分析试验,用2,3-二硝基水杨酸法(DNS)检测七味白术散总苷含量。结果表明:七味白术散总苷的最佳提取工艺为煎煮时间53 min、提取次数2次、料液比1∶13.7(g/mL),在此工艺下,七味白术散总苷的平均提取率提升至(0.71±0.01)%。
To optimize the extraction process of total glycosides of Qiweibaizhu Powder,the Box-Behnken response surface methodology was used in the three factors and three levels analysis,taking the extraction rate of total glycosides of Qiweibaizhu Powder as the response value,and decoction time,the number of extractions and solid-liquid ratio as the variables.The total glycoside content of Qiweibaizhu Powder was detected by 2,3-dinitrosalicylic acid method(DNS).The optimal extraction process for total glycosides of Qiweibaizhu Powder was:decocting time 53 min,extracting twice and solid-liquid ratio 1∶13.7(g/mL).Under such conditions,the average extraction rate of total glycosides of Qiweibaizhu Powder was about(0.71±0.01)%.
作者
唐圆
黄莉莉
谭周进
谢果珍
TANG Yuan;HUANG Li-li;TAN Zhou-jin;XIE Guo-zhen(Hunan University of Traditional Chinese Medicine,Changsha 410208,PRC)
出处
《湖南农业科学》
2021年第5期80-83,共4页
Hunan Agricultural Sciences
基金
国家自然科学基金青年基金(81804076)
湖南省自然科学基金青年基金(2020JJ5426)
湖南省研究生教育创新工程和专业能力提升项目(CX20200757)。
关键词
七味白术散总苷
Box-Behnken响应面法
提取率
优化
total glycosides of Qiweibaizhu Powder
Box-Behnken response surface methodology
extraction rate
optimization
作者简介
唐圆(1996-),女,土家族,湖南张家界市人,硕士研究生,研究方向为微生物与生化药学;通信作者:谢果珍。
