摘要
目的:基于网络药理学探讨淫羊藿-五味子药对治疗非酒精性脂肪肝的作用靶点及机制。方法:通过中药系统药理学数据库与分析平台(TCMSP)、中医药综合数据库(TCMID)获取淫羊藿和五味子的化学成分和靶点,并构建成分-靶点网络,其中化学成分的筛选条件为口服生物利用度(OB)≥30%和类药性(DL)≥0.18。通过GenCards和OMIM数据库筛选出淫羊藿-五味子治疗非酒精性脂肪肝的潜在作用靶点。利用Cytoscape软件构建疾病-成分-靶点之间网络图,进一步进行基因本体(GO)富集分析和京都基因和基因组百科全书(KEGG)富集分析。结果:共筛选出药效成分31个,药物与疾病作用交集靶点69个,涉及109条通路,显示“淫羊藿-五味子”可能通过作用于IL6、AKT1、CXCL8等关键靶点,主要参与转录正调控DNA模板、免疫应答、细胞对肿瘤坏死因子的反应等生物学过程,从而调节AGE-RAGE、NAFLD等通路,达到治疗作用。结论:通过中药网络药理学,初步探讨出“淫羊藿-五味子”治疗非酒精性脂肪肝的作用机制,为下一步实验验证提供基础。
Objective:To explore the action target and mechanism of drug pairs Herba Epimedii-Fructus Schisandrae Chinensis in the treatment of nonalcoholic fatty liver based on network pharmacology.Methods:The chemical components and targets of Herba Epimedii-Fructus Schisandrae Chinensis were obtained from TCMSP and TCMID databases,and the component-target network was constructed.Among them,the screening conditions of chemical components were oral bioavailability(OB)≥30%and drug-like properties(DL)≥0.18.The potential targets of Herba Epimedii-Fructus Schisandrae Chinensis in the treatment of non-alcoholic fatty liver were screened through GenCards and OMIM databases.Cytoscape software was used to construct a network diagram between disease-components-targets,and we further performed gene ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis.Results:A total of 31 efficacy components,69 drug and disease intersection targets,involving 109 pathway,were obtained,showing that Herba Epimedii-Fructus Schisandrae Chinensis may works by acting on vital targets such as IL6,AKT1,CXCL8.They mainly involved in the transcriptional positive regulation of DNA template,immune response,cell response to tumor necrosis factor and other biological processes,thereby regulating AGE-RAGE,NAFLD and other pathways to achieve therapeutic effects.Conclusion:This study preliminarily explores the mechanism of drug pairs Herba Epimedii-Fructus Schisandrae Chinensis in the treatment of non-alcoholic fatty liver through the pharmacology of traditional Chinese medicine network,to provide a basis for the next step of experimental verification.
作者
宋昌梅
杲春阳
付燕来
张晋
陈宝贵
SONG Changmei;GAO Chunyang;FU Yanlai;ZHANG Jin;CHEN Baogui(Treatment Center,Xiyuan Hospital,Chinese Academy of Chinese Medicine,Beijing 100091,China;Chen Precious Wuqing Traditional Chinese Medicine Studio,Tianjin University of Traditional Chinese Medicine,Tianjin 301700,China)
出处
《世界中医药》
CAS
2021年第14期2067-2072,共6页
World Chinese Medicine
基金
第四批全国优秀临床人才项目(国中医药人教发〔2018〕24)
全国名中医传承工作室建设项目(国中医药办人教函〔2018〕119号)。
关键词
淫羊藿-五味子
温阳化气
非酒精性脂肪肝
经验药对
作用机制
靶点
通路
网络药理学
Herba Epimedii-Fructus Schisandrae Chinensis
Warm yang to form qi
Nonalcoholic fatty liver disease
Experiential couplet medicinals
Mechanism of action
Target
Pathways
Network pharmacology
作者简介
宋昌梅(1994.03—),女,硕士研究生在读,研究方向:中医药治未病研究,E-mail:1040222033@qq.com;通讯作者:张晋(1969.03—),女,博士,主任医师,研究方向:中医药治未病研究,E-mail:13911893296@163.com。
