摘要
Photoacoustic microscopy(PAM)has become a premier microscopy tool that can provide the anatomical,functional,and molecular information of animals and humans in vivo.However,conventional PAM systems suffer from limited temporal and/or spatial resolution.Here,we present a fast PAM system and an agent-free localization method based on a stable and commercial galvanometer scanner with a custom-made scanning mirror(L-PAM-GS).This novel hardware implementation enhances the temporal resolution significantly while maintaining a high signal-to-noise ratio(SNR).These improvements allow us to photoacoustically and noninvasively observe the microvasculatures of small animals and humans in vivo.Furthermore,the functional hemodynamics,namely,the blood flow rate in the microvasculature,is successfully monitored and quantified in vivo.More importantly,thanks to the high SNR and fast B-mode rate(500 Hz),by localizing photoacoustic signals from captured red blood cells without any contrast agent,unresolved microvessels are clearly distinguished,and the spatial resolution is improved by a factor of 2.5 in vivo.LPAM-GS has great potential in various fields,such as neurology,oncology,and pathology.
基金
supported by the MSIT(Ministry of Science and ICT),Korea,under the“ICT Consilience Creative program”(IITP-2018-2011-1-00783)
supervised by the IITP(Institute for Information&Communications Technology Promotion)
supported by the National Research Foundation of Korea(NRF)grant funded by the Korean government(MSIT)(No.NRF-2019R1A2C2006269)
supported by the Pioneer Research Center Program through the National Research Foundation(NRF)funded by the Ministry of Science and ICT(NRF-2017M3C1A3037762)
Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(No.2017R1D1A1B03030087).
作者简介
Correspondence:Chulhong Kim,chulhong@postech.edu;Jongbeom Kim,contributed equally;Jin Young Kim,contributed equally。
