摘要
N6-甲基腺嘌呤(N6-methyladenosine,m^(6)A)是真核生物中普遍存在的RNA修饰。m^(6)A修饰在甲基化酶、去甲基化酶和结合蛋白的动态调控下,参与RNA剪接、出核、翻译等多种RNA代谢过程。近年研究表明,在卵母细胞发育成熟和精子发生等生殖过程中,RNA m^(6)A修饰发挥不可或缺的作用。在卵母细胞成熟过程中,m^(6)A动态调控网络通过参与RNA代谢过程,影响染色体、纺锤体组装,妨碍减数分裂进程,进而使卵泡发育受阻。颗粒细胞中m^(6)A水平异常引起的颗粒细胞凋亡与早发性卵巢功能不全(POI)、多囊卵巢综合征(PCOS)等卵巢功能损害的疾病相关。在男性生殖系统中,m^(6)A相关酶缺陷引起小鼠精子活力降低、数量和形态异常,人类弱精子症患者精液中m^(6)A水平升高。人工智能等新技术与m^(6)A测序技术的结合为未来m^(6)A动态网络的探索提供了新的方向。
N6-methyladenosine(m^(6)A)is the most common RNA modification in eukaryotic cells.Under the dynamic regulation of methylase,demethylase and binding protein,m^(6)A modification is involved in RNA metabolism processes,such as RNA splicing,nucleation and translation and so on.In recent years,many studies have shown that the RNA m^(6)A modification plays an indispensable role in the reproductive processes,such as oocyte maturation and spermatogenesis.During oocyte maturation,the dynamic regulation network of m^(6)A affects the assembly of chromosomes and spindles,interferes with meiosis and impedes the development of follicles.In granulosa cells,apoptosis of granulosa cells caused by the abnormal m^(6)A is related to POI and PCOS.In the male reproductive system,the deficiency of m^(6)A-related enzyme could damage the motility,number and morphology of sperm in mice,while the m^(6)A level in semen was increased in patients with asthenospermia.The combination of new technologies such as artificial intelligence and m^(6)A sequencing technology provides a new direction for the exploration of m^(6)A dynamic network in the future.
作者
张萌卉
刘笑聪(审校)
郭艺红(审校)
ZHANG Meng-hui;LIU Xiao-cong;GUO Yi-hong(The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)
出处
《国际生殖健康／计划生育杂志》
CAS
2021年第4期306-309,共4页
Journal of International Reproductive Health/Family Planning
作者简介
通信作者:郭艺红,E-mail:13613863710@163.com。
