摘要
[目的]旨在研究丝蛋白溶液的浓度、流量、是否载药对丝蛋白微球生成速率与粒径的影响。[方法]使用基于微加工技术制作的PDMS(聚二甲基硅氧烷)微流控芯片作为丝素蛋白微球的发生装置,通过调节微量注射泵改变丝素蛋白溶液的流量并使用高速摄影拍摄记录不同工况下微球的产生情况,最后使用Matlab进行数据处理分析。[结果]司盘80含量为4%的石蜡溶液与丝素蛋白溶液通过流动聚焦能稳定生成丝蛋白微球,生成的微球形状规则,粒径分布在15~35μm之间,经离心干燥后,丝蛋白微球粒径在2μm以内。[结论]固定连续相流量不变,微球的粒径、生成速率与分散相流量具有正相关性;分散相浓度增大,微球粒径减小,生成频率增大;当分散相加入药物span80之后,浓度增大,微球粒径减小,生成频率增大,微球具有很好的载药性。
[Objective]To study the influence of the concentration,flow rate,and the effect of adding drugs on the rate and particle size of silk protein microspheres.[Method]The PDMS(polydimethylsiloxane)microfluidic chip made based on micromachining technology was used as a device for generating silk fibroin microspheres.Changed the flow rate of the silk fibroin solution by adjusting the micro syringe pump and utilized high-speed photography to record the production of microspheres under different working conditions.Finally,Matlab was used for data processing and analysis.[Result]Paraffin solution containing 4%Span80 and silk fibroin solution can stably generate silk fibroin microspheres through flow focusing.And the generated microspheres are of regular shape,with particle size distribution between 15-35μm.After they were centrifuged and dried,the size of silk protein microspheres was within 2μm.[Conclusion]The flow rate of the fixed continuous phase remains unchanged,and the particle size and generation rate of the microspheres have a positive correlation with the flow rate of the dispersed phase;As the concentration of the dispersed phase increases,the particle size of the microspheres decreases and the frequency of generation increases;When the drug span80 was added to the dispersed phase,the concentration increases,the particle size of the microspheres decreases,and the frequency of generation increases.The microspheres have good drug-carrying properties.
作者
韩博
李芬
贾月梅
连小洁
田海平
HAN Bo;LI FenJIA Yue-mei;LIAN Xiao-jie;TIAN Hai-ping(Department of National Experimental Teaching Demonstration Center of Mechanics,Taiyuan University of Technology,Taiyuan 030024,China;Department of Mechanicai and Vehicle Engineering,Taiyuan University of Technology,Taiyuan 030024,China;Department of Biomedical Engineering,Taiyuan University of Technology,Taiyuan 030024,China)
出处
《生物技术》
CAS
2021年第3期300-305,共6页
Biotechnology
基金
国家自然科学基金-青年科学基金项目(11802195,11502157)
山西省应用基础研究项目青年科技研究项目(201801D221027)。
关键词
流动聚焦
丝素蛋白微球
粒经
生成频率
flow focusing
silk protein microsphere
partical size
generate frequency
作者简介
韩博(1995-),男,山西省临汾市人,硕士,研究方向:实验流体力学,E-mail:hanbotyut@163.com;通讯作者:李芬(1980-),女,博士,讲师,研究方向:生物医学工程,E-mail:lifen01@tyut.edu.cn。
