摘要
时间分辨荧光分析技术是一种检测固定波长处荧光随时间变化的技术,它以镧系元素及其复合物作为荧光标记物,具有高效率、高特异性、超高灵敏度等特点。食品中一些低浓度的污染物由于具有蓄积毒性和高致癌性等,对检测方法的灵敏度提出很高的要求,时间分辨荧光分析技术可以满足此类污染物检测中对高灵敏度的要求。该文介绍这一技术的原理和特点并对其在食品污染物检测中的应用进行综述。
Time-resolved fluorescence analysis is a technique to detect the change of fluorescence with time at fixed wavelength.It uses lanthanide and its complexes as fluorescent markers.It has the characteristics of high efficiency,high specificity and high sensitivity because of this principle.Because of the accumulation toxicity and high carcinogenicity of some low concentration pollutants in food,the sensitivity of the detection method is very high,and the time-resolved fluorescence analysis technology can meet the requirements of high sensitivity in the detection of these pollutants.This paper introduced the principle and characteristics of this technology and summarized its application in food contamination detection.
作者
孙坤秀
胡志刚
成玉梁
于航
谢云飞
姚卫蓉
郭亚辉
钱和
SUN Kun-xiu;HU Zhi-gang;CHENG Yu-liang;YU Hang;XIE Yun-fei;YAO Wei-rong;GUO Ya-hui;QIAN He(School of Food Science and Technology,State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214000,Jiangsu,China;Wuxi Children's Hospital Medical Laboratory,Wuxi 214000,Jiangsu,China)
出处
《食品研究与开发》
CAS
北大核心
2021年第13期214-218,共5页
Food Research and Development
关键词
时间分辨荧光分析
荧光标记物
高灵敏
食品安全
食品污染物
time-resolved fluorescence analysis
fluorescent marker
high sensitivity
food safety
food con-taminant
作者简介
孙坤秀(1997-),女(汉),本科,研究方向:食品安全;通信作者:郭亚辉(1988-),男(汉),副教授,博士,研究方向:食品安全检测。
