摘要
为获得高温高效分解茶渣的菌株。利用高温特殊生境分离方法,从废弃茶渣中分离到有分解茶渣作用的5株菌,其中Fb菌株产生的蛋白酶、纤维素酶的活力均最高。经形态学、生理生化特性以及生长特性等研究,并克隆Fb菌株的16S rDNA基因序列和gyrB基因序列,进行系统发育学分析。结果表明,Fb属贝莱斯芽孢杆菌(Bacillus velezensis),可耐受55℃的高温。最适生长条件为:装液量30 mL、摇床转速分120 r/min、培养温度42~45℃、初始pH 5.0~7.0、培养时间16 h、盐度1.0%~6.0%。本研究首次在茶渣中分离筛选出贝莱斯芽抱杆菌,为茶渣开发研究提供理论依据。
In order to obtain strains which can decomposing tea residue in high-temperature and with high efficiency,we used the high temperature special habitat separation method,and isolated 5 bacteria strains that could decompose tea residues.Among them,the protease and cellulase produced by the Fb strain had the highest activity.After studying the morphology,physiological and biochemical characteristics and growth characteristics of the Fb strain,the 16S rDNA gene sequence and the gyrB gene sequence of the strain were cloned,and the phylogenetic analysis was carried out.The results showed that Fb belonged to Bacillus velezensis,which could tolerate the high temperature of 55℃.The optimal growth condition of the strain was:the filling volume of 30 mL,the rotation speed of the shaking table of 120 r/min,the incubation temperature of 42-45℃,the initial pH of 5.0-7.0,the incubation time of 16 h,and the salinity of 1.0%-6.0%.For the first time,Bacillus velezensis is isolated and screened out of tea residue,and the study could provide a theoretical basis for the development and research of tea residue.
作者
缪伏荣
陈鑫珠
邱华玲
刘景
Miao Furong;Chen Xinzhu;Qiu Hualing;Liu Jing(Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350013)
出处
《中国农学通报》
2021年第18期109-116,共8页
Chinese Agricultural Science Bulletin
基金
福建省农业科学院自由探索科技创新项目“茶渣饲料资源化利用的关键技术研究”(ZYTS2019022)。
关键词
茶渣
贝莱斯芽孢杆菌
分离
筛选
tea residue
Bacillus velezensis
isolation
screening
作者简介
第一作者:缪伏荣,男,1969年出生,福建霞浦人,副研究员,硕士,主要从事饲料资源和特种微生物的研究与应用。通信地址:350013福州市晋安区新店镇埔垱村100号福建省农科院牧医所动物营养研究室,Tel:13067107348,E-mail:miaofr@sina.com;通讯作者:刘景,男,1967年出生,福建长乐人,副研究员,主要从事饲料资源的研究。通信地址:350013福州市晋安区新店镇埔垱村100号福建省农科院牧医所动物营养研究室,Tel:0591-87869491,E-mail:fjliuj@163.com。
