摘要
【目的】研发适用于酿酒酵母菌剂生产的高密度低成本培养基。【方法】以酿酒酵母JH301为对象菌,以糖蜜为基础培养基,采用单因素试验筛选适宜的碳源、氮源、无机盐和天然产物增殖因子,通过U16(88)均匀试验优化培养基成分。【结果】研究筛选出适宜的碳源为棉籽糖、葡萄糖和海藻糖,氮源为胰蛋白胨,无机盐为磷酸二氢钾,天然产物增殖因子为酒糟多肽、大米糖化液和大豆多糖。获得优化培养基配方为:在可溶性固形物含量为10%的糖蜜中添加酒糟多肽23.72 g·L^(−1)、大米糖化液44.15 g·L^(−1)、大豆多糖31.88 g·L^(−1)、棉籽糖22.45 g·L^(−1)、葡萄糖13.97 g·L^(−1)、海藻糖15.00 g·L^(−1)、胰蛋白胨27.25 g·L^(−1)、磷酸二氢钾3.00 g·L^(−1),调节pH至4.5±0.2。酵母细胞增殖培养生物量可达1.48×108 cfu·mL^(−1),比基础糖蜜培养基提高了1个数量级。【结论】酒糟多肽中富含多种对酿酒酵母细胞增殖起促进作用的小分子多肽成分,作为天然产物添加到糖蜜培养基中可显著提高酵母产量,是一种来源丰富、低成本的优良复合营养源,相关研究内容可为高活力酵母菌剂制备提供思路和参考依据。
【Objective】An optimized low-cost medium for high-density culture of Saccharomyces cerevisiae was formulated.【Method】S.cerevisiae JH301 was cultured on the basic molasses media of varied compositions to determine the optimal carbon source,nitrogen source,inorganic salt,and natural growth promoters for the yeast preparation in a single factor test.Optimization models using different statistical analyses were compared for the formulation finalization.【Result】From a U16(88)uniform experiment,the optimized medium formulation was determined to apply the carbon source that included glucose,cottonseed sugar,and trehalose,tryptone for the nitrogen source,K2HPO4 as the inorganic salt,and the natural growth promoters that consisted of distiller's grains polypeptide,rice saccharification solution,and soybean polysaccharide.The finalized formula called for 23.72 g·L^(−1) distiller's grains polypeptide,44.15 g·L^(−1) rice saccharification solution,31.88 g·L^(−1) soybean polysaccharide,22.45 g·L^(−1) cottonseed sugar,13.97 g·L^(−1) glucose,15.00 g·L^(−1) trehalose,27.25 g·L^(−1) tryptone,and 3.00 g·L^(−1) K2HPO4,in addition to 10%soluble solids molasses.The resulting yeast biomass reached 1.48×108 cfu·mL^(−1),which was one order of magnitude higher than the culture obtained from the basic molasses medium.【Conclusion】Rich in small molecular polypeptides capable of promoting the cell proliferation of S.cerevisiae,the natural distiller's grains polypeptide was formulated in the medium to yield a low-cost preparation for the yeast.
作者
苏昊
梁璋成
林晓姿
何志刚
邓冬莲
林晓婕
SU Hao;LIANG Zhangcheng;LIN Xiaozi;HE Zhigang;DENG Donglian;LIN Xiaojie(Institute of Agricultural Engineering Technology,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China;Fujian Key Laboratory of Agricultural Products(Food)Processing,Fuzhou,Fujian 350003,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2021年第5期610-618,共9页
Fujian Journal of Agricultural Sciences
基金
福建省科技计划公益类专项(2019R1032-5、2018R1014-5)。
关键词
酿酒酵母
糖蜜
酒糟多肽
高密度培养
增殖培养基
Saccharomyces cerevisiae
molasses
distiller's grains polypeptide
high-density culture
proliferation medium
作者简介
苏昊(1984−),男,博士,助理研究员,主要从事食品发酵与酿造方面研究(E-mail:suhaoxp@163.com);通信作者:何志刚(1964−),男,研究员,主要从事农产品贮藏与加工方面研究(E-mail:njgzx@163.com)。
