摘要
[目的]探究红桦组织培养各个阶段的最佳培养基配比,建立红桦组织培养体系,为红桦良种选育、定向培育及遗传转化等研究提供理论支持。[方法]以红桦休眠芽为外植体,研究外植体最佳消毒灭菌方法,6-BA、NAA浓度对休眠芽萌发、增殖培养的影响以及基本培养基类型、IBA和蔗糖浓度对不定根诱导的影响。[结果]表明:完整的红桦组织培养体系为:(1)以红桦休眠芽为外植体,先用70%酒精消毒30 s,无菌水冲洗3~4次,再用0.1%HgCl_(2)灭菌8 min,无菌水冲洗5~6次获得无菌材料;(2)休眠芽萌发最适宜培养基配方为WPM+1.5 mg·L^(-1) 6-BA+0.1 mg·L^(-1) NAA,外植体萌发率为83.33%;(3)增殖培养所需最佳激素配比为WPM+1.5 mg·L^(-1) 6-BA+0.02 mg·L^(-1) NAA,增殖系数达到4.77,健康指数达到2.45;(4)不定根诱导最佳配比为WPM+0.8 mg·L^(-1) IBA,生根率达100%,根健康指数为2.61;所有培养基均附加30 g·L^(-1)蔗糖与7g·L^(-1)琼脂。[结论]红桦以WPM为基本培养基,添加不同浓度的6-BA、NAA、IBA,可成功进行各阶段的培养,最终建立完整的红桦器官发生途径再生体系,再生植株移植成活率在80%以上。
[Objective]To explore the optimal medium ratio at each stage of the tissue culture of Betula albo-sinensis, to establish the tissue culture system of B. albo-sinensis, and provide theoretical support for the studies on species selection of superior varieties, directional cultivation and genetic transformation of B. albo-sinensis. [Method]Using dormant buds of B. albo-sinensis as explants to study the optimal disinfection and sterilization method of explants,the effect of 6-BA and NAA concentrations on the germination of dormant buds and proliferation culture, and the effects of basic medium types, IBA and sucrose concentrations on the induction of adventitious root. [Result]The optimal tissue culture system of B. albo-sinensis established in this study is as follows.(1) Using B. albo-sinensis dormant buds as explants, and sterilized with 70% alcohol for 30 seconds and washed with sterile water for 3-4 times,then sterilized with 0.1% HgCl_(2) for 8 minutes and washed with sterile water for 5-6 times to obtain sterile materials.(2) The optimal medium for the germination of dormant buds is WPM basic medium with 1.5 mg·L^(-1) 6-BA and 0.1 mg·L^(-1) NAA, the germination rate of explants is 83.33%.(3) The best proliferation culture is WPM adding 1.5 mg·L^(-1) 6-BA and 0.02 mg·L^(-1) NAA, with a proliferation coefficient of 4.77 and a health index of 2.45.(4) The most suitable medium for adventitious root induction is WPM supplemented with 0.8 mg·L^(-1) IBA, the rooting rate is100%, and the root health index is 2.61. All the media are supplemented with 30 g·L^(-1) sucrose and 7 g·L^(-1) agar.[Conclusion]With WPM as the basic medium and different concentrations of 6-BA, NAA and IBA added, the culture of B. albo-sinensis at all stages could be successfully carried out, and a complete regeneration system of organogenesis generation pathway is established. The transplant survival rate of regenerated plants is higher than 80%.
作者
付雪宁
高洪治
申耀荣
王永康
姜在民
蔡靖
FU Xue-ning;GAO Hong-zhi;SHEN Yao-rong;WANG Yong-kang;JIANG Zai-min;CAI Jing(College of Forestry,Northwest A&F University,Yangling 712100,Shaanxi,China;Taoping Forest Farm,Xiaolongshan Forestry Experimental Bureau,Longnan 742200,Gansu,China;Matoutan Forestry Bureau,Baoji 721006,Shaanxi,China;College of Life Science,Northwest A&F University,Yangling 712100,Shaanxi,China)
出处
《林业科学研究》
CSCD
北大核心
2021年第3期194-200,共7页
Forest Research
基金
国家重点研发计划子课题“红桦高效培育技术研究”(2017YFD0600603-02)。
关键词
红桦
器官发生
植物生长调节剂
植株再生
Betula albo-sinensis Burk
organogenesis
plant growth regulators
plant regeneration
作者简介
通讯作者:蔡靖,女,教授.主要研究方向:植物资源开发利用,森林生态.E-mail:cjcaijing@163.com。
