摘要
采用液质联用(HPLC-MS)的方法检测菌株Cupriavidus sp.DT-1降解2-羟基吡啶(2-HP)的代谢产物.并用三亲结合、荧光定量PCR(q-PCR)方法评价降解菌对3,5,6-三氯-2-吡啶酚(TCP)污染土壤的修复效果.结果表明,菌株可以进一步降解2-HP,依次生成尼古丁蓝、马来酰胺酸和反丁烯二酸,直至转化成菌株DT-1生长的碳源.接种菌株DT-1对污染土壤中TCP的降解起到较大的促进作用,2组试验土壤中TCP(50mg/kg)降解率分别为94.4%和86.7%,未接种菌株的土壤中TCP降解率仅为20.4%和28.4%.带有绿色荧光蛋白基因gfp标记的菌株DT-1-gfp可在土壤中存活35d以上,并对TCP污染土壤的细菌群落丰度有显著的恢复作用.
Cupriavidus sp.DT-1 was a 3,5,6-trichloro-2-pyridinol(TCP)-degrading strain which could transform TCP to 2-hydroxypyridine(2-HP).Liquid-mass spectrometry(HPLC-MS)was used to detect the degradation products of 2-HP,And the methods of triparetal conjugation,quantitative real-time PCR(q-PCR)were used to evaluate the remediation effect of TCP-contaminated soils by the degrading-bacterium.Results showed that strain DT-1 was able to further degrade 2-HP,and sequentially produced nicotine blue,maleamic acid and fumaric acid,until it was transformed into the carbon source that could support the growth of strain DT-1.Pilot experiment showed that inoculation of strain DT-1 remarkably accelerated the elimination of TCP in soils.The degradation rates of TCP in inoculated soils were 94.4%and 86.7%,while those in uninoculated soils were 20.4%and 28.4%,respectively.Green fluorescent protein encoding gene gfp harbored strain DT-1-gfp could survive in soils for more than 35d.The results of q-PCR showed that inoculation of strain DT-1-gfp significantly improved the recovery of bacterial community abundance in the TCP-contaminated soils.
作者
陆鹏
周慧
袁梦
LU Peng;ZHOU Hui;YUAN Meng(Anhui Key Laboratory of Molecular Enzymology and Major Disease Mechanism research,College of Life Sciences,Anhui Normal University,Wuhu 241000,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2021年第6期2780-2787,共8页
China Environmental Science
基金
安徽省高校自然科学研究项目(KJ2020A0084)。
作者简介
责任作者:陆鹏(1986-),男,江苏徐州人,讲师,博士,主要研究方向为环境微生物学.发表论文8篇,lupeng_2007@126.com。
