摘要
目的探讨T2DM患者血清miR‐320表达与DR的相关性。方法选取2018年5月至2020年5月于孝感市中心医院内分泌和风湿科住院治疗的T2DM患者262例,根据DR诊断及分期标准将患者分为非DR组(non‐DR,n=83),非增殖性DR组(非增殖组,n=85),增殖性DR组(增殖组,n=94)。比较各组患者一般资料和主要生化指标,实时荧光定量PCR术检测各组血清miR‐320表达。结果增殖组年龄高于non‐DR、非增殖组(P<0.05)。non‐DR、非增殖和增殖组DM病程、FPG、HbA1c、HOMA‐IR依次升高(P<0.05)。非增殖组eGFR、miR‐320水平高于增殖组(P<0.05)。Pearson相关分析显示,T2DM患者血清miR‐320表达与eGFR呈正相关(P<0.05),与DM病程、TG、LDL‐C、FPG、HbA1c和HOMA‐IR呈负相关(P<0.05)。Logistic回归分析显示,DM病程、TG、LDL‐C、FPG、HbA1c、HOMA‐IR、eGFR和miR‐320表达是T2DM患者发生DR的影响因素。T2DM患者血清miR‐320表达量预测发生DR时,曲线下面积为0.788(95%CI 0.734,0.842),当miR‐320表达量为0.90时,灵敏度为63.13%,特异度为91.57%。结论T2DM患者发生DR时血清miR‐320表达降低,且与DR病程进展及糖脂代谢指标相关,是发生DR的危险因素,可能是预测T2DM患者发生DR的潜在生物学标志物。
Objective To investigate the correlation between the expression of serum miR‐320 and diabetic retinopathy(DR)in type 2 diabetes mellitus patients(T2DM).Methods A total of 262 T2DM patients who were hospitalized in Xiaogan Central Hospital were selected from May 2018 to May 2020.According to DR diagnosis and staging criteria,the patients were divided into non‐DR group(n=83)and DR group(n=179).The DR group was divided into non‐proliferative subgroup(n=85)and proliferative subgroup(n=94).The general information of patients and main biochemical indicators were collected.The expression of miR‐320 in serum was detected by real‐time fluorescence quantitative PCR.Results The age,course of diabetes and the levels of FPG,HbA1c and HOMA‐IR in the non‐DR,non‐proliferative DR subgroup and DR subgroup showed an increasing trend(P<0.05),while the levels of eGFR and miR‐320 presented a downward trend(P<0.05).Pearson correlation analysis showed that the expression level of miR‐320 in the serum of T2DM patients was negatively correlated with the duration of diabetes,TG,LDL‐C,FPG,HbA1c and HOMA‐IR(P<0.05),and positively correlated with eGFR(P<0.05).Logistic regression analysis showed that the duration of diabetes,TG,LDL‐C,FPG,HbA1c,HOMA‐IR,eGFR and the expression level of miR‐320 were influencing factors for DR(P<0.05).When the expression level of miR‐320 in serum of T2DM patients was used to predict the occurrence of DR,the area under the curve was 0.788(95%CI 0.734,0.842).When the cutoff value of miR‐320 was 0.90,the sensitivity was 63.13%and the specificity was 91.57%.Conclusion The expression level of miR‐320 in serum when DR occurs in T2DM patients is decreased,and is related to the progression of DR and glucose and lipid metabolism indicators.It may be a potential biomarker for predicting the occurrence of DR in T2DM patients.
作者
孙志兵
杨娟
SUN Zhibing;YANG Juan(Department of Endocrinology and Rheumatology,Xiaogan Central Hospital,Xiaogan 432000,China)
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2021年第5期344-348,共5页
Chinese Journal of Diabetes
基金
湖北省2019年卫健委联合基金(WJ2019H386)。
作者简介
通信作者:杨娟,Email:249038988@qq.com。
