摘要
毛蕊花糖苷是由咖啡酸、羟基酪醇、葡萄糖和鼠李糖组成的苯乙醇苷类衍生物,广泛存在于多种天然药用植物中,具有抗炎、抗菌、抗病毒、抗肿瘤、抗氧化、镇痛、神经保护、改善性功能、免疫调节和改善细胞记忆等多方面的生物活性。然而,植物提取来源的毛蕊花糖苷存在含量低、环境污染问题,无法实现绿色可持续的规模化生产。利用合成生物学成功构建了一系列植物天然产物的微生物细胞工厂,为高效生产毛蕊花糖苷提供了新思路。综述了毛蕊花糖苷细胞工厂的研究现状,包括苯乙醇苷生物合成关键代谢途径解析、关键基因元件挖掘和优化、关键前体物的合成等方面,以期为毛蕊花糖苷的生物合成奠定基础。
Acteoside is a natural product widely found in many plants,which is composed of caffeic acid,hydroxytyrosol, glucose and rhamnose, including anti-inflammatory, antibacterial, antiviral, anti-tumor,antioxidant,analgesic,neuroprotective,improving sexual function,immunomodulatory,and memory protective activities. However,traditional extraction of acteoside and its derivatives from plants have some problems such as low content,low extraction efficiency and environmental pollution. In recent years,the study of heterologous synthesis of plant natural products has been successfully constructed by the rapid development of synthetic biology,which provides a new idea for the efficient production of acteoside. The recent advances of acteoside cell factory which includes the key metabolic biosynthesis elucidations of phenylethanoid glycoside,mining and optimization of key gene components,synthesis of key precursors were summarized.
作者
翟君叶
成旭
孙泽敏
李春
吕波
ZHAI Jun-ye;CHENG Xu;SUN Ze-min;LI Chun;LV Bo(School of Chemistry and Chemical Engineering,Beijing Institute of Technology,Beijing 102488,China;School of Life Sciences,Beijing Institute of Technology,Beijing 100081,China;Department of Chemical Engineering,Tsinghua University,Beijing 100084,China)
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2021年第5期94-104,共11页
China Biotechnology
关键词
苯乙醇苷
毛蕊花糖苷
途径解析
生物合成
Phenylethanoid glycoside
Acteoside
Pathway analysis
Biosynthesis
作者简介
通讯作者:吕波,电子信箱:lv-b@bit.edu.cn。
