摘要
【目的】为规模化生产上紫苏褐斑病的防治提供参考。【方法】以感染褐斑病的紫苏叶片为试验材料,采用形态学和分子生物学相结合的方法鉴定紫苏褐斑病病原菌,在此基础上采用抑菌圈法对抑菌剂进行室内毒力测定。【结果】紫苏褐斑病病原菌为链格孢属真菌(Alternaria Nees)。72%甲霜锰锌、70%代森锰锌和69%烯酰锰锌的500~800倍稀释液均可抑制链格孢属真菌的生长,3种抑菌剂的抑菌率均与药品浓度呈正相关关系,72%甲霜锰锌500倍液的抑菌效果最强,抑菌率为73.4%;72%甲霜锰锌EC_(50)为0.76μg/mL;70%的代森锰锌EC_(50)为0.19μg/mL;69%烯酰锰锌EC_(50)为0.16μg/mL。【结论】紫苏褐斑病病原菌为链格孢属真菌,3种抑菌剂对紫苏褐斑病均有防治效果,69%烯酰锰锌对菌丝生长的整体抑制效果最好。
【Objective】The present paper aimed to provide a reference for prevention and control of brown spot in scale production of Perilla frutescens.【Method】The pathogen isolated from Perilla frutescens leaves infected with brown spot was identified by morphology and molecular biology method and then the indoor toxicity of three fungicides against the identified pathogen was measured by an inhibition zone method.【Result】The isolated pathogen was identified as Alternaria.72%Metalaxyl-mancozeb×500-800 times,70%Mancozeb×500-800 times and 69%Dimethomorph-Mancozeb×500-800 times all could inhibit mycelial growth of Alternaria.There was a positive correlation between antibacterial rate and application concentration in three fungicides.The antibacterial rate of 72%Metalaxyl-mancozeb×500 times was up to 73.4%(the highest).EC_(50)of 72%Metalaxyl-mancozeb,70%Mancozeb and 69%Dimethomorph-Mancozeb was 0.76,0.19 and 0.16μg/mL respectively.【Conclusion】The pathogen infecting Perilla frutescens brown spot is identified as Alternaria and three tested fungicides all have the prevention and control effect on Perilla frutescens brown spot.69%Dimethomorph-Mancozeb has the overall antibacterial effect on mycelial growth of Alternaria.
作者
赵刘赵
商志伟
杨小华
罗充
ZHAO Liu-zhao;SHANG Zhi-wei;YANG Xiao-hua;LUO Chong(College of Life Sciences,Guizhou Normal University,Guizhou Guiyang 550000,China;Rape Institute,Guizhou Academy of Agricultural Sciences,Guizhou Guiyang 550006,China;Agricultural Bureau Bijiang Bureau of Agriculture and Rural Affairs,Guizhou Tongren 554300,China)
出处
《西南农业学报》
CSCD
北大核心
2021年第5期1024-1028,共5页
Southwest China Journal of Agricultural Sciences
基金
贵州省科技支撑计划项目“紫苏‘叶籽双收’型种质资源创制”[黔科合支撑(2018)2346]
贵州省农业科学院青年基金项目“药用型紫苏种质资源的筛选”[黔农科院青年基金(2018)03]。
关键词
紫苏
褐斑病
分子鉴定
抑菌剂
抑菌率
Perilla frutescens
Brown spot
Molecular identification
Fungicide
Antibacterial rate
作者简介
赵刘赵(1995-),男,河南济源人,在读顿士,研究方向:紫苏病虫害及生理生化,E-mail:761156094@qq.com;承罗充(1970-),男,侗族,贵州铜仁人,教授,从事植物生理学研究,E-mail:gzluochong@163.com。
