摘要
目的:探讨苓桂术甘汤加味联合卡托普利对心力衰竭大鼠血清B型钠尿肽(BNP)、内皮素-1(ET-1)、半胱氨酸天冬氨酸蛋白酶3(Caspase-3)水平的影响。方法:选取40只SPF级SD大鼠,连续1周腹腔注射异丙肾上腺素,建立慢性心力衰竭大鼠模型,左室射血分数(LVEF)≤60%则造模成功。将造模成功的SD大鼠随机分为心衰组、中药组、西药组、联合组进行观察。结果:光镜下观察各组大鼠心肌病理形态学变化,与心衰组比较,给予药物治疗的三组心肌病理学形态有所改善,血清BNP、ET-1、Caspase-3水平降低,LVEF升高,心脏质量指数下降。结论:苓桂术甘汤加味联合卡托普利能减轻慢性心力衰竭大鼠心肌细胞的病理损伤,降低血清BNP、ET-1、Caspase-3水平及心脏质量指数,提高LVEF水平,改善心功能。
Objective:To investigate the effects of modified Linggui Zhugan prescription combined with captopril on serum levels of BNP,ET-1 and Caspase-3 in rats with chronic heart failure.Methods:40 SPF SD rats were selected and intraperitoneally injected with isoproterenol for one week to establish the rat model of chronic heart failure.LVEF≤60%the model was successfully established.SD rats were randomly divided into heart failure group,Chinese medicine group,western medicine group,combined group for observation.Results:The pathological morphological changes of myocardium were observed under light microscope.Compared with the heart failure group,the pathological morphological changes of myocardium in the three groups treated with drugs were improved with decreased serum levels of BNP,ET-1 and Caspase-3,increased LVEF and decreased heart mass index.Conclusion:Modified Linggui Zhugan prescription combined with captopril can reduce the pathological damage of cardiomyocytes in rats with chronic heart failure,reduce serum BNP,ET-1,Caspase-3 levels and heart mass index,increase LVEF level,decrease,and improve cardiac function.
作者
张燕飞
张博
陈苏宁
ZHANG Yanfei;ZHANG Bo;CHEN Suning(Shengjing Hospital of China Medical University,Shenyang 110004,China)
出处
《陕西中医》
CAS
2021年第6期701-704,共4页
Shaanxi Journal of Traditional Chinese Medicine
基金
辽宁省自然科学基金资助项目(MB87)。
关键词
慢性心力衰竭
B型尿钠肽
内皮素-1
半胱氨酸天冬氨酸蛋白酶3
苓桂术甘汤加味
大鼠
Chronic heart failure
B-type natriuretic peptide
Endothelin-1
Cysteine aspartate specific protease 3
Modified Linggui Zhugan prescription
Rats
