摘要
目的探讨基于高通量基因表达数据库(Gene expression Omnibus,GEO数据库)分析配对盒基因8(PAX8)在非小细胞肺癌(NSCLC)病人癌组织中的表达情况及与预后的关系。方法利用在线工具Oncomine分析PAX8在NSCLC与正常肺组织的表达情况,基于GEO数据库下载相关芯片数据(GSE77803),收集156例NSCLC组织的基因表达谱及相关临床数据,采用中位数法将其分为PAX8低表达组(n=78)和PAX8高表达组(n=78)。分析PAX8的表达与病人性别、年龄、病理类型、T分期、N分期和术后复发情况之间的关系;采用生存分析PAX8的表达与病人预后生存之间的关系;采用基因本体(GO)注释分析PAX8基因的功能,采用KEGG PATHWAY分析PAX8可能参与的信号通路。结果TCGA Lung 2数据集共检测了1537个样本,其中正常肺组织样本390例,肺癌组织样本共721例,血液样本420例,无意义样本6例。与正常肺组织相比,PAX8在肺腺癌和肺鳞癌中表达水平明显升高(P<0.05);基于GEO数据库中GSE77803(GPL570)数据集分析,NSCLC癌组织中PAX8的表达水平与病人的T分期、N分期和是否复发有关(P<0.05),生存分析显示,癌组织中PAX8低表达病人的总生存率为52.94%,中位生存期为54.6个月,显著高于PAX8高表达病人(34.35%,48.6个月)(log-rankχ~2=4.609,P=0.032)。PAX8低表达病人无病生存率为42.50%,中位生存期为51.7个月,显著高于PAX8高表达病人(28.71%,40.8个月)(log-rankχ~2=5.712,P=0.017);GO注释和KEGG分析显示,PAX8可能参与调节NSCLC发生发展相关的信号通路的表达。结论PAX8蛋白在肺腺癌和肺鳞癌中均存在异常高表达,PAX8低表达NSCLC病人具有更高的生存率。
Objective To explore expression of paired box gene 8(PAX8)in cancer tissues of non-small cell lung cancer(NSCLC)patients and its relationship with prognosis based on Gene expression Omnibus(GEO)database.Methods The online tool Oncomine was applied to analyze expression of PAX8 in NSCLC and normal lung tissues.Based on GEO database,related chip data(GSE77803)were downloaded.The gene expression profiles and related clinical data of 156 NSCLC tissues in 156 cases were collected and assigned into PAX8 low-expression group(n=78)and PAX8 high-expression group(n=78)by median method-The relationship between expression of PAX8 and gender,age,pathological type,T stage,N stage,postoperative recurrence was analyzed.The survival analysis was applied to analyze relationship between PAX8 expression and prognosis survival of patients.Gene Ontology(GO)annotations were applied to analyze PAX8 gene function.The signal pathways that PAX8 might participate in were analyzed by Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway.Results There were 1537 samples detected by The Cancer Genome Atlas(TCGA)Lung 2 dataset,including 390 cases with normal lung tissues,721 cases with lung cancer tissues,420 cases with blood and 6 cases with meaningless-Compared with normal lung tissues,the level of PAX8 expression was significantly increased in lung adenocarcinoma and lung squamous carcinoma(P<0.05).Based on GSE77803(GPL570)dataset analysis in GEO database,the level of PAX8 expression in NSCLC tissues was related with T stage,N stage and recurrence of patients(P<0.05).Survival analysis showed that overall survival rate and median survival time of patients with PAX8 low expression in cancer tissues was significantly higher than those with PAX8 high expression(52.94%,54.6 months vs.34.35%,48.6 months)(log-rankχ~2=4.609,P=0.032).The disease-free survival rate and median survival time of patients with PAX8 low expression were significantly higher than those with PAX8 high expression(42.50%,51.7 months vs.28.71%,40.8 months)(log-rankχ~2=5.712,P=0.017).GO annotations and KEGG analysis showed that PAX8 might be involved in signaling pathways which regulated occurrence and development of NSCLC.Conclusion PAX8 protein is abnormally highly expressed in lung adenocarcinoma and lung squamous carcinoma.The survival rate is higher in NSCLC patients with PAX8 low expression.
作者
渠茂林
QU Maolin(Laboratory Department,Hohhot Mongolian Medical Hospital of Traditional Chinese Medicine,Hohhot,Inner Mongolia Autonomous Region 010010,China)
出处
《安徽医药》
CAS
2021年第6期1169-1172,共4页
Anhui Medical and Pharmaceutical Journal
