摘要
以酒红球盖菇R-4菌株为试材,采用微生物平板培养法,研究了12种氮源和8种碳源对酒红球盖菇生长的影响,以期为酒红球盖菇培养提供参考依据。结果表明:酒红球盖菇菌丝生长对氮源和碳源的选择差异不显著(P>0.05),均能利用试验所选的12种氮源和8种碳源,当酵母膏作为氮源和葡萄糖作为碳源时酒红球盖菇的菌落长势较优,菌丝生长速度较快。葡萄糖当作最优碳源时酒红球盖菇菌落生长速率、菌落直径、生长速度、菌丝生长指数分别是9.75 mm·d^(-1)、50.22 mm、1.41 mm·d^(-1)和46.73,酵母膏当作最优氮源时酒红球盖菇菌落生长速率、菌落直径、生长速度、菌丝生长指数分别是14.12 mm·d^(-1)、52.54 mm、2.10 mm·d^(-1)和43.56,由此得出,酒红球盖菇生长的最优碳源是葡萄糖,最优氮源是酵母膏。
R-4 strains of Stropharia rugosoannulata were used as test materials, microbial plate culture method was used, the effects of 12 kinds of nitrogen sources and 8 kinds of carbon sources on the growth of R-4 strains were studied, in order to provide reference for the cultivation of R-4 strains.The results showed that there was no significant difference in the selection of nitrogen and carbon sources for the mycelial growth of Stropharia rugosoannulata(P>0.5),12 nitrogen sources and 8 carbon sources selected in the experiment could be used.When yeast extract was used as nitrogen source and glucose was used as carbon source,the colony growth of Stropharia rugosoannulata was better and the mycelial growth rate was faster.When glucose was used as the optimal carbon source,the colony growth rate,colony diameter,growth rate and hyphal growth index were 9.75 mm ·d^(-1),50.22 mm,1.41 mm·d^(-1) and 46.73,respectively.When yeast extract was used as the optimal nitrogen source,The value of indicator described above were 14.12 mm ·d^(-1),52.54 mm,2.10 mm·d^(-1) and 43.56,respectively.The results showed that glucose and the yeast extract was the best carbon source and nitrogen source respectively for the growth of Stropharia rugosoannulata.
作者
马令法
何淑玲
杨敬军
孙耶宾
唐龙娇
黄晶
MA Lingfa;HE Shuling;YANG Jingjun;SUN Yebin;TANG Longjiao;HUANG Jing(College of Chemistry and Bioengineering,Guangxi Normal Univercity for Nationlities,Chongzuo,Guangxi 532200;Guangxi Institute of Feature Fungus Resources Research,Chongzuo,Guangxi 532200)
出处
《北方园艺》
CAS
北大核心
2021年第8期106-111,共6页
Northern Horticulture
基金
广西崇左市科技局重大专项资助项目(崇科发[2018]4号)
广西壮族自治区教学改革工程重点资助项目(2019JGZ152)
广西民族师范学院科研经费资助项目(2017FG002)
广西科技厅重点研发计划资助项目(2018AB44049)。
关键词
酒红球盖菇
培养基
菌丝
菌落
Stropharia rugosoannulata
culture medium
hyphae
colony
作者简介
第一作者:马令法(1975-),男,博士,副教授,现主要从事食药用菌和药用植物资源与利用等研究工作。E-mail:lingfa2008@163.com;责任作者:何淑玲(1975-),女,博士,副教授,现主要从事药用植物资源与利用等研究工作。E-mail:heshuling2010@163.com.
