摘要
针对化妆品在使用过程中会使人体皮肤出现过敏、炎症等问题,使用高效液相色谱-串联质谱法(HPLC-MS)快速筛查化妆品中8种违禁药物(米诺地尔,雌三醇,螺内酯,坎利酮,雌酮,雌二醇,己烯雌酚,黄体酮)。将化妆品样品经过一定体积比的甲醇-水混合溶液提取后,利用HLB固相萃取柱进行净化处理,采用色谱柱分离复溶后的样液,以浓度为10 mmol/L的甲醇-水作为流动相进行梯度洗脱,并对8种化妆品违禁药物进行定性和定量分析,检测出8种化妆品的回收率为80%~96%,相对标准偏差为3.7%~8.2%,可满足化妆品违禁药物的筛查要求,提高了化妆品中违禁药物快速筛查准确度。
In response to people’s skin allergies and inflammation during the process of using cosmetics,highperformance liquid chromatography-tandem mass spectrometry(HPLC-MS)was used to quickly screen 8 prohibited drugs(minoxidil,estriol,spironolactone,canrenone,estrone,estradiol,diethylstilbestrol,progesterone prog)in cosmetics.The cosmetic samples were extracted by methanol water mixed solution with a certain volume ratio.HLB solid phase extraction column is used to purify cosmetic samples.The chromatographic column was used to separate the reconstituted sample solution.Methanol-water with a concentration of 10 mmol/L was used as the mobile phase for gradient elution.8 kinds of prohibited drugs in cosmetics were analyzed qualitatively and quantitatively.Experiments have detected that the recovery rate of 8 kinds of cosmetics are between 80%and 96%.The relative standard deviations of 8 kinds of cosmetics are between 3.7%and 8.2%,which can meet the screening requirements of prohibited drugs in cosmetics and improve the accuracy of rapid screening of prohibited drugs in cosmetics.
作者
赵峥嵘
ZHAO Zheng-rong(College of Food Engineering,Henan Vocational College of Agricultural,Zhengzhou,Henan 451450,China)
出处
《日用化学工业》
CAS
北大核心
2021年第5期463-467,共5页
China Surfactant Detergent & Cosmetics
基金
河南省科技厅科技攻关计划项目(182102110185)。
关键词
高效液相色谱法
化妆品
违禁药物
快速筛查
high performance liquid chromatography-tandem mass spectrometry
cosmetics
prohibited drugs
rapid screening
作者简介
赵峥嵘(1979-),女,河南牟县人,讲师,电话:13783652200,E-mail:33417935@qq.com。
