摘要
目的研究阳桃根Averrhoa carambola L化学成分及其体外抗肿瘤活性。方法阳桃根75%乙醇提取物采用硅胶、Sephadex LH-20进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用MTT法评价其体外抗肿瘤活性。结果从中分离得到15个化合物,分别鉴定为3,4,5-三甲氧基苯甲酸(1)、8,9-dehydropellitorine(2)、7-O-feruloylorientin(3)、2-乙酰基-3,5-二羟基-苯乙酸甲酯(4)、对羟基苯丙酸(5)、1,6-desoxypipoxide(6)、isolug-randoside(7)、liriotulipiferine(8)、2,5,7,9-四羟基-4-甲氧基-9,10-二氢菲(9)、6,3′,4′-trihydroxyaurone(10)、2-羟基-1-(4-羟基-3-甲氧基-苯基)-丙基-1-酮(11)、diasyringaresinol(12)、邻苯二甲酸二正丁酯(13)、isosilychristin(14)、pyrrolezanthine-6-ethyl ether(15)。化合物7对MCF7、SKOV-3细胞,化合物8对MCF7、HeLa、HT29细胞,化合物12对HeLa、SKOV-3、HT29细胞的IC_(50)值分别为(47.93±2.86)、(46.86±2.71),(39.84±2.65)、(41.83±2.67)、(42.65±2.71),(35.67±2.57)、(46.58±2.73)、(33.86±2.65)μmol/L。结论所有化合物均为首次从该植物中分离得到,化合物7~8、12具有体外抗肿瘤活性。
AIM To study the chemical constituents from Averrhoa carambola L and their anti-tumor activity in vitro.METHODS The 75%ethanol extract from A.carambola was isolated and purified by silica and Sephadex LH-20,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their anti-tumor activity in vitro was evaluated by MTT method.RESULTS Fifteen compounds were isolated and identified as 3,4,5-trimethoxybenzoic acid(1),8,9-dehydropellitorine(2),7-O-feruloylorientin(3),2-acetyl-3,5-dihydro-phenylpropionic acid(4),p-hydroxyphenylpropionic acid(5),1,6-desoxypipoxide(6),isolugrandoside(7),liriotulipiferine(8),2,5,7,9-tetrahydro-4-methoxy9,10-dihydrophenyl(9),6,3′,4′-trihydroxyaurone(10),2-hydroxyl-1-(4-hydroxy-3-methoxyphenyl)-propyl-1-ketone(11),diasyringaresinol(12),din-butyl phthalate(13),isosilychristin(14),pyrrolezanthine-6-ethyl ether(15).The IC_(50) values of compound 7 on MCF7,SKOV-3 cells,compound 8 on MCF7,HeLa,HT29 cells,and compound 12 on HeLa,SKOV-3,HT29 cells are(47.93±2.86),(46.86±2.71),(39.84±2.65),(41.83±2.67),(42.65±2.71),(35.67±2.57),(46.58±2.73),(33.86±2.65)μmol/L,respectively.CONCLUSION All the compounds are isolated from this plant for the first time.Compounds 7-8,12 have antitumor activities in vitro.
作者
卫晓娅
郑新华
李清正
高华山
王继红
马素丽
WEI Xiao-ya;ZHENG Xin-hua;LI Qing-zheng;GAO Hua-shan;WANG Ji-hong;MA Su-li(Medical College,Pingdingshan University,Pingdingshan 467000,China)
出处
《中成药》
CAS
CSCD
北大核心
2021年第5期1206-1211,共6页
Chinese Traditional Patent Medicine
基金
河南省科技厅科技攻关项目(202102310478)
河南省高等学校重点科研项目(20B350006)。
关键词
阳桃根
化学成分
分离鉴定
抗肿瘤活性
Averrhoa carambola L
chemical constituents
isolation and identification
anti-tumor activity
作者简介
卫晓娅(1982-),女,硕士,讲师,从事医学教育与药理学研究。Tel:(0375)9454267,E-mail:gu3doctor@163.com;通信作者:郑新华(1970-),女,硕士,教授,从事肿瘤病理研究。Tel:(0375)9454263,E-mail:doctor777zy@163.com。
