摘要
该研究以西藏琵蝎(Scorpiops tibetanus)为研究对象,采用Illumina高通量测序技术及生物信息学等方法,对其肠道细菌多样性、群落组成及16S rRNA功能进行研究。结果表明,西藏琵蝎肠道细菌多样性丰富,群落结构复杂,优势门为厚壁菌门(Firmicutes,57.82%)和变形杆菌门(Proteobacteria,28.25%),优势目为芽孢杆菌目(Bacillales,55.19%),优势属为短芽孢杆菌属(Brevibacillus,24.63%)和解硫胺素芽孢杆菌属(Aneurinibacillus,22.91%),优势种为波茨坦短芽孢杆菌(Brevibacillus borstelensis)和解硫胺素芽孢杆菌(Aneurinibacillus aneurinilyticus),以上两种细菌均为首次在动物肠道中发现;16S rRNA功能预测结果显示,西藏琵蝎肠道细菌功能基因丰富,包含丰富的初级代谢和次级代谢通路。该研究揭示了西藏琵蝎肠道细菌的多样性及群落组成,为西藏琵蝎相关研究提供了一定的参考。
The diversity,community composition and 16S rRNA function of enterobacteria in Scorpiops tibetanus were studied by high-throughput sequencing and bioinformatics.The results showed that the diversity of intestinal bacteria was rich and the community structure was complex.The dominant phylum was Firmicutes(57.82%)and Proteobacteria(28.25%),the dominant order was Bacillales(55.19%),and the dominant genera were Brevibacillus(24.63%)and Aneurinibacillus(22.91%).The dominant species were Brevibacillus borstelensis and Aneurinibacillus aneurinilyticus,both of which were found in animal intestines for the first time.The results of 16S rRNA function prediction showed that there were abundant functional genes in intestinal bacteria of S.tibetanus,including genes related to rich primary and secondary metabolic pathways.The study reveals the bacterial diversity and community composition in the gut of S.tibetanus,and has reference value for the related research.
作者
向礼波
何敏
郑恒
XIANG Li-bo;HE Min;ZHENG Heng(Institute of Plant Protection and Soil Fertilizer,Hubei Academy of Agricultural Sciences,Wuhan 430000,Hubei,China;College of Science,Tibet University,Lhasa 850000,Tibet,China)
出处
《生命科学研究》
CAS
CSCD
2021年第2期131-135,139,共6页
Life Science Research
基金
国家自然科学基金资助项目(31701834)。
作者简介
向礼波(1982—),男,湖北武汉人,副研究员;通信作者:郑恒(1990—),男,陕西商洛人,硕士研究生,主要从事青藏高原生物多样性与分子进化研究,E-mail:635881104@qq.com。
