摘要
目的:对黄芪种子抗菌肽提取条件进行优化,提高提取率并测定抑菌率。方法:以黄芪种子为材料,使用双指数响应面分析法对其提取条件进行优化,使用96孔板法测定抑菌率。结果:黄芪种子抗菌肽的最佳提取条件:浸提液为EDTA缓冲液,料液比为1:5 g/mL,浸提时间为16.5 h,硫酸铵质量百分比浓度为82%。此工艺下,黄芪种子抗菌肽的抑菌率可达80.3%,比优化前(75.6%)提高了4.7%;提取率可达79.6%,较优化前(67.2%)提高了12.4%。结论:采用双指数响应面分析法对黄芪种子抗菌肽的提取条件的优化有效,并且抑菌率也有很大提升。
Objective:To optimize the extraction conditions of antimicrobial peptides from Astragalus seeds,improve the extraction rate and determine the antibacterial rate.Methods:Using Astragalus seeds as material,the extraction conditions were optimized by double exponential response surface analysis,and the inhibition rate was determined by 96-well plate culture.Results:The optimal extraction conditions of antimicrobial peptides from Astragalus seeds were as follows:the extract was EDTA buffer,the material-liquid ratio was 1:5 g/mL,the extraction time was 16.5 h,and the ammonium sulfate saturation was 82%.The antibacterial rate of Astragalus seed antimicrobial peptides can reach 80.3%,which is 4.7% higher than that before optimization (75.6%).The extraction rate is 79.6%,which is an increase of 12.4% compared to before preoptimization (67.2%).Conclusion:The double exponential response surface analysis is effective to optimize the extraction conditions of antimicrobial peptides from Astragalus seeds,and the antibacterial rate is also greatly improved.
作者
张弘弛
刘瑞
柴帅
李慧
杨阳
ZHANG Hongchi;LIU Rui;CHAI Shuai;LI Hui;YANG Yang(College of Life Science,Datong University,Applied Biotechnology Institute,Datong 037009,China)
出处
《食品科技》
CAS
北大核心
2021年第3期212-219,共8页
Food Science and Technology
基金
山西省应用基础研究计划项目(201901D111313)
山西省高等学校科技创新项目(2020L0469)
大同市重点研发计划项目(2017015)。
关键词
黄芪种子
抗菌肽
提取条件
抑菌率
提取率
Astragalus seeds
antimicrobial peptides
extraction conditions
antibacterial rate
extraction rate
作者简介
张弘弛(1980-),男,山西大同人,博士,副教授,研究方向为天然药物化学;通信作者:刘瑞。
