摘要
目的探讨FAM72D在肺腺癌(LUAD)中的表达变化及其与预后的关系,并进行生物学机制的预测。方法从TCGA获取原始数据,采用Kaplan-Meier生存分析、单变量Cox分析、多变量Cox分析、表达差异分析、临床相关性分析FAM72D在评估LUAD患者预后中的作用;采用基因集富集分析预测FAM72D的潜在作用机制,并利用TIMER数据库分析FAM72D与LUAD免疫浸润之间的相关性。结果FAM72D的高表达与LUAD患者的预后不良有关(P<0.05),是LUAD患者的独立预后指标。与正常肺组织比较,FAM72D表达在LUAD组织中明显上调,FAM72D高表达与高龄呈负相关,与男性、临床分期、T分期呈正相关,差异有统计学意义(P<0.05)。GSEA结果显示细胞周期、同源重组、剪接体、RNA降解、DNA复制、P53通路等相关基因集明显富集于FAM72D高表达。TIMER数据库分析显示,LUAD患者FAM72D表达水平与B细胞、巨噬细胞渗透水平呈负相关,与中性粒细胞的渗透水平呈正相关,差异有统计学意义(P<0.05)。结论FAM72D可作为LUAD早期诊断和预测预后的生物标志物。
Objective To explore the relationship between the expression of FAM72D in lung adenocarcinoma(LUAD)and prognosis,and to made the predictions of biological mechanism.Methods The raw data were obtained from TCGA.The role of FAM72D in evaluating the prognosis of LUAD was analyzed by adopting the Kaplan-Meier survival analysis,univariate Cox analysis,multivariate Cox analysis,expression difference analysis and clinical correlation.The potential action mechanism of FAM72D was predicted by adopting the gene set enrichment analysis(GSEA),and the correlation between FAM72D and LUAD immune infiltration was analyzed by using the TIMER database.Results The high expression of FAM72D was associated with the poor prognosis(P<0.05),which was an independent prognostic index for the patients with LUAD.The FAM72D expression in LUAD was significantly up-regulated,and its high expression was negatively correlated with the advanced age,and positively correlated with male,clinical stage and T stage,and the differences were statistically significant(P<0.05).The GSEA results indicated that the gene sets such as the cell cycle,homologous recombination,spliceosome,RNA degradation,DNA replication and P53 signaling pathway were significantly enriched in the FAM72D high-expression.The TIMER database analysis showed that the expression level of FAM72D in the patients with LUAD was negatively correlated with the infiltration level of B cells and macrophages,positively correlated with the infiltration level of neutrophils,and the differences werestatistically significant(P<0.05).Conclusion FAM72D can serve as a biomarker for early diagnosis and prognosis prediction of LUAD.
作者
郑泰浩
李明
张晓月
彭媛
郭政军
黄秀
凌丰宇
杨镇洲
ZHENG Taihao;LI Ming;ZHANG Xiaoyue;PENG Yuan;GUO Zhengjun;HUANG Xiu;LENG Fengyu;YANG Zhenzhou(Department of Oncology,Affiliated Yongchuan Hospital of Chongqing Medical University,Chongqing 402160,China;Tumor Center,Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China)
出处
《重庆医学》
CAS
2021年第7期1081-1086,共6页
Chongqing medicine
基金
国家自然科学基金项目(81972851)
重庆市自然科学基金博士后科学基金项目(cstc2019jcyj-bshX0056)。
作者简介
郑泰浩(1987-),主治医师,硕士,主要从事肺癌分子机制研究;通信作者:杨镇洲,E-mail:yangzz@cqmu.edu.cn。
