摘要
目的:评价经典名方中半夏"汤洗"炮制的科学性和可行性,为相关经典名方的开发提供参考。方法:基于古代"汤洗"炮制建立水洗半夏炮制方法,通过单因素试验优选工艺条件,测定炮制前后水分、灰分、浸出物,对其炮制前后质量变化、总酸含量(以琥珀酸计)以及高效液相色谱法(HPLC)指纹图谱进行比对,并进行家兔眼刺激试验,以评价水洗半夏上述指标及刺激性变化情况。结果:水洗半夏炮制方法为加4倍量80℃热水反复清洗10次,洗至水清澈,切开断面透心,口尝无麻感或者微麻。水洗半夏的水分平均值9.34%,灰分平均值1.71%,浸出物平均值4.22%,质量下降率平均值7.49%,总酸质量分数下降率平均值43.31%,水洗炮制前后HPLC指纹图谱发现各成分均有所减少,但并无新增色谱峰,其中9号峰(腺苷)减少较为明显。家兔眼刺激试验结果表明水洗半夏浸液未出现明显眼结膜刺激性,水洗后毒性明显降低。结论:建立的半夏"汤洗"炮制方法稳定可行,半夏水洗后的浸提液对家兔眼结膜无刺激性。
Objective: To evaluate the scientificity and feasibility of processing of Pinelliae Rhizoma by hot water washing(Tangxi),and to provide reference for the development of related famous classical formulas. Method: Processing method of Pinelliae Rhizoma washed by hot water was established based on ancient Tangxi processing method,and the process conditions were optimized by single factor tests. The weight,moisture, ash, extract, total acid(calculated by succinic acid) contents and high performance liquid chromatography(HPLC) fingerprint of Pinelliae Rhizoma were compared before and after processing. In addition,the rabbit eye irritation test was conducted to evaluate the toxicity changes. Result: The processing method of Pinelliae Rhizoma washed by hot water was as following:washed by 4 times the amount of hot water at 80 ℃ for 10 times until clear water,transfused cross-section after incision,no or slight numbness in the mouth. The average moisture,ash,extract contents of Pinelliae Rhizoma washed by hot water were 9.34%,1.71% and 4.22%,respectively. After being processed,the decline rates of weight and total acid content of Pinelliae Rhizoma were 7.49% and 43.31%. The HPLC fingerprint of Pinelliae Rhizoma before and after washing showed a decrease in all components,but there was no new chromatographic peak,and peak 9(adenosine)reduced significantly. The results of rabbit eye irritation test showed that there was no obvious eye conjunctival irritation after washing,indicating that the toxicity of Pinelliae Rhizoma decreased obviously after washing.Conclusion: The established method of Pinelliae Rhizoma by Tangxi processing is stable and feasible,the aqueous extract of Pinelliae Rhizoma has no obvious eye conjunctival irritation after washing.
作者
李东影
崔凯茜
孟贺
朱晓光
易剑平
LI Dong-ying;CUI Kai-xi;MENG He;ZHU Xiao-guang;YI Jian-ping(National Engineering Research Center for Development of Compound Chinese Medicine,Beijing Zhongyan Tongrentang Pharmaceutical Research and Development Co.Ltd.,Beijing 100079,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2021年第7期127-133,共7页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
水洗
半夏
炮制
质量
眼刺激试验
高效液相色谱法(HPLC)
指纹图谱
water washing
Pinelliae Rhizoma
processing
quality
eye irritation test
high performance liquid chromatography(HPLC)
fingerprint
作者简介
第一作者:李东影,硕士,工程师,E-mail:lidongyingl2272006@126.com;通信作者:易剑平,博士,副研究员,Tel:010-87632543,E-mail:jianping74@163.com。
