摘要
二甲酸钾(KDF)为抗生素的新型替代品,但在牲畜饲养中还未大量普及。采用水热法自制P型分子筛(Zeolite P),负载KDF分散在羧甲基纤维素(CMC)溶液中,与FeCl_(3)交联,利用凝聚法制备壳聚糖-羧甲基纤维素-P型分子筛-二甲酸钾pH敏感水凝胶抗菌微球。通过FT-IR,TGA和SEM分析可知,壳聚糖(CS)和CMC通过离子键形成结构稳定的聚电解质复合物,Zeolite P镶嵌缠绕在CMC基质中。溶胀差异性表明水凝胶微球具有高pH敏感性,可以适用不同pH条件下的持续给药。缓释动力学研究表明:抗菌微球对KDF具有一定的缓释作用,且遵循一级动力学释放模型和Higuchi模型。体外抗菌实验发现,抗菌液浓度为24 mg/mL和48 mg/mL时对大肠杆菌和金黄色葡萄球菌有显著的抗菌性,可以有效地抑制细菌的生长。
Potassium diformate(KDF)is a novel substitute for antibiotics,which is not widely used in livestock production.The P-type molecular sieve(Zeolite P)was prepared by hydrothermal method with loading KDF and dispersed in the carboxymethyl cellulose(CMC)solution.The dispersed CMC mixed solution was added dropwise to the FeCl_(3) solution for cross-linking.Chitosan-carboxymethyl cellulose-Zeolite P-potassium diformate pH-sensitive hydrogel antibacterial microspheres were prepared by coacervation method.FT-IR,TGA and SEM results show that CS and CMC form the structurally stable polyelectrolyte complex through ionic bonds,and Zeolite P is included in the CMC matrix.The difference in swelling study indicates that the hydrogel microspheres have high pH-sensitivity and can be applied for sustained-release under different pH value.The slow-release kinetics study shows that antimicrobial microspheres have a certain sustained-release effect on KDF.The first-order kinetic model and the Higuchi model fits well with release data.In vitro antibacterial experiments,the antibacterial microspheres display significant antibacterial property against Escherichia coli and Staphylococcus aureus in the concentration of the antibacterial solution at 24 mg/mL and 48 mg/mL.Antibacterial microspheres can effectively inhibit the growth of bacteria,and the antimicrobial microsphere model provides a theoretical basis for the use of KDF.
作者
郭小炜
李玉妍
王秀丽
杨忠鑫
陈南春
解庆林
GUO Xiao-wei;LI Yu-yan;WANG Xiu-li;YANG Zhong-xin;CHEN Nan-chun;XIE Qing-lin(School of Materials Science and Engineering,Guilin University of Technology,Guilin 541004,Guangxi,China;School of Chemical and Biological Engineering,Guilin University of Technology,Guilin 541006,Guangxi,China;Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area,Guilin University of Technology,Guilin 541006,Guangxi,China)
出处
《材料工程》
EI
CAS
CSCD
北大核心
2021年第4期142-149,共8页
Journal of Materials Engineering
基金
国家自然科学基金项目(51564008,41662005)
广西科技攻关项目(桂科攻11107003-4)
广西自然科学基金项目(2015GXNSFAA139094,2016GXNSFAA380014)。
关键词
水凝胶
P型分子筛
PH敏感
缓释
抗菌
hydrogel
Zeolite P
pH-sensitivity
sustained-release
bacteriostatic
作者简介
通讯作者:陈南春(1962-),男,教授,研究方向为环境矿物材料,联系地址:广西壮族自治区桂林市桂林理工大学材料学与工程学院(541004),E-mail:cnc@glut.edu.cn;通讯作者:王秀丽(1964-),女,高级工程师,研究方向为药物化学、生物工程、沸石分子筛抗菌性能、天然植物,联系地址:广西壮族自治区桂林市桂林理工大学化学与生物工程学院(541006),E-mail:610064853@qq.com。
