摘要
岩藻多糖是大型褐藻门植物中的一种天然杂多糖,为进一步开发其生物活性,笔者以其为原料,采用酶法降解的方式制备了不同聚合度的岩藻寡糖(FOS)混合物,并采用电喷雾质谱(ESI-MS)对降解得到的寡糖产物进行结构表征,采用ABTS法、DPPH法及羟基自由基清除法对寡糖混合物的抗氧化活性进行了评价,以植物乳杆菌(Lactobacillus plantarum)和嗜酸乳杆菌(Lactobacillus acidophilus)为对象表征了寡糖混合物的益生活性。电喷雾质谱表征分析获得了岩藻寡糖的组成:一系列低聚合度的硫酸化岩藻寡糖,主要包括[Fuc_(1-4)SO_(3)Na-Na]^(-)、[Fuc_(4-5)(SO_(3)Na)_(2)-Na]^(-)和[Fuc_(4-5)(SO_(3)Na)_(3-4)-Na]^(-)等。益生活性和抗氧化活性研究表明,岩藻寡糖可有效促进植物乳杆菌和嗜酸乳杆菌的增殖,并具有良好的ABTS^(+)·、DPPH·和·OH清除能力。岩藻寡糖质量浓度为10 mg/mL时,对ABTS^(+)·、DPPH·和·OH的清除率分别达到98.83%、94.91%和70.49%。
Fucoidian is a kind of natural heteropolysaccharides in large brown algae.To develop its biological activity,fucoidian was used as raw material,and fucoidan oligosaccharides(FOS)were prepared by enzymatic degradation of fucoidan and structurally identified by electrospray ionization mass spectrometry(ESI-MS).The antioxidant activity of the FOS was investigated by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)method,1,1-diphenyl-2-picrylhydrazyl(DPPH)method and hydroxyl radical scavenging method.In addition,the probiotic activity of FOS was determined by Lactobacillus plantarum and Lactobacillus acidophilus.ESI-MS analysis indicated that the mixture included series of FOS with low degree of depolymerization such as[Fuc_(1-4)SO_(3)Na-Na]^(-),[Fuc_(4-5)(SO_(3)Na)_(2)-Na]^(-)and[Fuc_(4-5)(SO_(3)Na)_(3-4)-Na]^(-).The antioxidant and probiotic experiments suggested that FOS could effectively enhance the proliferation of Lactobacillus plantarum and Lactobacillus acidophilus.Furthermore,the in vitro antioxidant activity experiment showed that FOS at the concentration of 10 mg/mL had strong ABTS^(+)·,DPPH·and·OH radical scavenging ability and the scavenging rates were 98.83%,94.91%and 70.49%,respectively.
作者
曹声生
朱本伟
高翔
王剑朋
杜媛媛
周婉婷
姜进举
周明柱
姜帅
CAO Shengsheng;ZHU Benwei;GAO Xiang;WANG Jianpeng;DU Yuanyuan;ZHOU Wanting;JIANG Jinju;ZHOU Mingzhu;JIANG Shuai(College of Food Science and Light Industry,Nanjing Tech University,Nanjing 211800,China;Qingdao Bright Moon Seaweed Group Co.,Ltd.,Qingdao 266400,China;Suqian Advanced Materials Institute,Nanjing Tech University,Suqian 223800,China)
出处
《生物加工过程》
CAS
2021年第2期156-161,共6页
Chinese Journal of Bioprocess Engineering
基金
国家自然科学基金(31401610)
宿迁市科技计划(现代农业)项目(L201906)
农业部海藻类肥料重点实验室开放课题(MAKLSF1808)。
关键词
岩藻寡糖
海洋生物资源
抗氧化活性
益生活性
自由基
嗜酸乳杆菌
植物乳杆菌
fucoidan oligosaccharides
structural characterization
antioxidant activity
probiotic activity
free radical
Lactobacillus acidophilus
Lactobacillus plantarum
作者简介
曹声生(1998—),男,河南南阳人,硕士研究生,研究方向:功能食品研究与开发;联系人:朱本伟,副教授,E-mail:zhubenwei@njtech.edu.cn。
