摘要
采用150 mmol·L^(-1)NaCl和100 mmol·L^(-1)NaHCO_(3)/Na_(2)CO_(3)(1∶1)混合溶液分别模拟盐和碱胁迫,对283份小麦种质资源进行萌发期盐碱胁迫试验,基于11项性状指标综合分析了小麦种质资源耐盐碱性特点。结果表明:在盐和碱胁迫下,11项指标值在283份小麦种质资源之间差异显著。NaCl胁迫下,胚芽长、胚芽鲜重和胚芽干重变幅较大;碱胁迫下,胚根长、胚根鲜重、胚根干重变幅较大。隶属函数值相关性分析表明,各性状之间存在不同程度的相关性,且盐碱胁迫下各性状之间的相关性存在差异。选取8个变异系数大的性状进行主成分分析,归纳为4个主成分,盐、碱胁迫累计贡献率分别为76.2%和83.59%。通过聚类分析,将盐、碱胁迫下283份小麦材料各划分为3类,筛选出10份耐盐碱型材料,分别为白芒麦、f106、和尚头、四方麦、轴包、中梁22、f78、蚰子麦、和尚麦、矮立多。该研究结果为小麦萌发期耐盐碱品种的培育耐及盐碱胁迫相关机理研究提供了参考依据。
Salt and alkali stress tests were carried out on 283 wheat germplasm resources at germination stage by using 150 mmol·L^(-1)NaCl and 100 mmol·L^(-1)NaHCO_(3)/Na_(2)CO_(3)mixture(1∶1)solutions to simulate salt and alkali stress,respectively.Saline-alkali tolerance characteristics of wheat germplasm resources were comprehensively analyzed based on 11 trait indexes.The results showed that there were significant differences between the 11 indexes for 283 wheat germplasm resources under salt and alkali stress.Under NaCl stress,the variations of germ length,germ fresh weight and germ dry weight were larger.Under alkali stress,radicle length,radicle fresh weight and radicle dry weight varied greatly.The correlation analysis of membership function values showed that there were different degrees of correlations among the different traits,and there were differences among the traits under saline-alkali stress.Eight traits with large coefficient of variation were selected for principal component analysis,which were summarized into four principal components.The cumulative contribution rates of salt and alkali stress were 76.2%and 83.59%,respectively.After cluster analysis,283 wheat materials under salt and alkali stress were divided into 3 categories,and 10 saline-alkali resistant materials were selected,which were Baimangmai,f106,Heshangtou,Sifangmai,Zhoubao,Zhongliang22,f78,Youzi,Heshangmai and Ailiduo.The results provided a reference for the cultivation of salt-alkali tolerant wheat during germination and research on the mechanism of saline-alkali stress in wheat.
作者
李媛媛
陈博
姚立蓉
翟雪婷
司二静
汪军成
马小乐
孟亚雄
王化俊
李葆春
杨亮
LI Yuanyuan;CHEN Bo;YAO Lirong;ZHAI Xueting;SI Erjing;WANG Juncheng;MA Xiaole;MENG Yaxiong;WANG Huajun;LI Baochun;YANG Liang(College of Life Sciences and Technology,Gansu Agricultural University,Lanzhou 730070,China;College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2021年第3期25-33,共9页
Journal of Agricultural Science and Technology
基金
国家自然科学基金项目(31860377)
国家现代农业产业技术体系项目(CARS-05-04B-2)
甘肃省科技支撑项目(1604NKC052-2)
甘肃省干旱生境作物学重点实验室开放基金项目(GSC5-2016-03)
甘肃省科技重大专项(17ZD2NA016)
甘肃农业大学盛彤笙科技创新基金项目(GSAU-STS-1513)。
关键词
小麦
萌发期
盐碱胁迫
wheat
germination period
saline alkali stress
作者简介
联系方式:李媛媛,E-mail:917023591@qq.com;通信作者:李葆春,E-mail:libc@gsau.edu.cn。
