摘要
目的探究特发性和先天性脊柱侧凸椎间盘终板软骨的钙结合蛋白S100和相关蛋白表达价值。方法回顾性分析2019年1~12月湘南学院附属医院收治的22例脊柱侧凸患者的病例资料,其中12例为特发性脊柱侧凸作为IS组,10例为先天性脊柱侧凸作为CS组,对椎间盘终板软骨中S100A8及热休克蛋白90(HSP90)以及钙调蛋白(Ca M)基因表达情况予以实时聚合酶链式反应(RT-PCR)法分析,比较2组内和2组间顶椎间盘与端椎间盘以及同一椎间盘凸侧凹侧的表达差异性。结果RT-PCR分析结果显示,IS组顶椎终板软骨中S100表达与端椎差异无统计学意义(P>0.05);CS组顶椎终板软骨中S100表达显著低于端椎,差异有统计学意义(P<0.05);IS组顶椎凹与端椎凹S100表达显著低于CS组,差异有统计学意义(P<0.05)。IS组顶椎终板软骨中HSP90表达与端椎差异无统计学意义(P>0.05);CS组HSP90在顶椎凹与端椎凹表达差异有统计学意义(P<0.05),在顶椎凸与端椎凸端软骨表达差异无统计学意义(P>0.05);IS组顶椎凹软骨中HSP90表达低于CS组,差异有统计学意义(P<0.05),2组在顶椎凸、端椎凹以及端椎凸中表达差异无统计学意义(P>0.05)。IS组顶椎终板软骨中Ca M表达与端椎差异无统计学意义(P>0.05);CS组顶椎凹软骨中Ca M表达低于端椎凹,差异有统计学意义(P<0.05),顶椎凸Ca M表达与端椎凸差异无统计学意义(P>0.05);IS组端椎终板及顶椎终板软骨中Ca M表达均显著高于CS组,差异有统计学意义(P<0.05)。结论S100、Ca M在IS与CS中表达呈现出明显差异性,可能参与了脊柱侧凸形成及发展,应予以高度重视。
Objective To investigate the expression value of calcium-binding protein S100 and related proteins in intervertebral disc endplate cartilage in patients with scoliosis. Methods A retrospective analysis in January to December 2019,Hospital Affiliated to Xiangnan University,22 cases of patients with scoliosis data of 12 cases of idiopathic scoliosis as IS group,10 cases with congenital scoliosis as CS group,using TRIzol method on two groups of cartilage endplate in total protein extraction,the disc in the cartilage endplate S100A8 and heat shock protein 90(HSP90) and calmodulin(CaM) gene expression in Western technical analysis,contrast the difference in expression. Results RT-PCR analysis showed that there was no statistical difference significance between the expression of S100 in the apical endplate cartilage of the IS group and the end vertebrae(P > 0. 05);the expression of S100 in the apical endplate cartilage of the CS group was significantly lower than that of the end vertebrae,and the difference was statistically significant(P < 0. 05);the expression of S100 in the apical and end vertebrae of the IS group was significantly lower than that of the CS group,the difference was statistically significant(P < 0. 05). There was no statistically significant difference between HSP90 expression in apical endplate cartilage and end-vertebral cartilage in the IS group(P > 0. 05). The expression of HSP90 in the apical and end vertebrae of the CS group was different statistically significant(P < 0. 05). There was no statistically significant difference in cartilage expression between apical and end vertebral protrusions(P > 0. 05). The expression of HSP90 in the apical foveal cartilage in the IS group was lower than that in the CS group,and the difference was statistically significant(P < 0. 05). The expression in the apical vertebral protrusion,end vertebral cavity and end vertebral protrusion was not statistically significant in the two groups(P > 0. 05). The expression of CaM in the apical endplate cartilage of the IS group was not significantly different from that of the end vertebrae(P > 0. 05);the CaM expression in the apical foveal cartilage of the CS group was lower than that of the end vertebral fovea,and the difference was statistically significant(P < 0. 05). The expression of CaM in apical vertebral convexity was not statistically different from that in apical vertebral convexity(P > 0. 05);the expression of CaM in endplate and apical endplate cartilage of IS group was significantly higher than that of CS group,and the difference was statistically significant(P < 0. 05). Conclusion S100 and CaM show significant differences in the expression of IS and CS,and may be involved in the formation and development of scoliosis,which should be paid close attention to.
作者
唐光
陈飞
李永斌
TANG Guang;CHEN Fei;LI Yong-bin(Departmen of Spinal Surgery,Hospital Affiliated to Xiangnan University,Chenzhou Hunan 423000,China;Departmen of Spinal Surgery,the Second Xiangya Hospital Affiliated to Central South University,Changsha Hunan 410000,China)
出处
《临床和实验医学杂志》
2021年第5期522-526,共5页
Journal of Clinical and Experimental Medicine
基金
湖南省科技创新项目(编号:1920001001660)。
作者简介
通讯作者:陈飞,E-mail:chenfei@aliyun.com。
