摘要
[目的]检测程序性死亡配体1(programmed death ligand 1,PD-1)、肿瘤浸润淋巴细胞(tumor infiltrating lymphocyte,TIL)和叉头转录因子P3(forkhead box P3,FoxP3)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达,分析其相关性及其与临床病理参数之间的关系。[方法]纳入2017年1月至2018年12月于徐州医科大学附属医院手术治疗的NSCLC患者669例,收集其手术切除的肿瘤组织,制成组织微阵列(TMA),采用免疫组化EnVision方法检测肿瘤细胞PD-L1、肿瘤间质TIL和Treg的表达水平。[结果]TIL在NSCLC不同组织学类型、性别、吸烟、淋巴结转移、脉管侵犯、TNM分期中存在统计学差异(P<0.01)。PD-L1、FoxP3^(+)Treg在各临床病理参数中均无统计学差异。PD-L1与TIL呈正相关(P<0.01),FoxP3^(+)Treg与TIL呈正相关(P<0.01)。肿瘤低分化、肿瘤>3cm、淋巴结转移、脉管侵犯、TNMⅢ/Ⅳ期、PD-L1和FoxP3^(+)Treg高表达的NSCLC患者术后无复发生存期更短(P<0.05);鳞癌、男性、吸烟、肿瘤低分化、肿瘤>3cm、淋巴结转移、脉管侵犯、TNMⅢ/Ⅳ期、TIL极低浸润的NSCLC患者术后总生存期更短(P<0.05)。[结论]PD-L1、FoxP3^(+)Treg高表达、低淋巴细胞浸润的NSCLC患者预后不良。PD-L1、FoxP3^(+)Treg可作为NSCLC术后复发/转移的独立预后指标。
[Objective]To investigate the expression of programmed death ligand 1(PD-L1),tumor infiltrating lymphocyte(TIL)and Foxp3+regulatory T cell(FoxP3^(+)Treg)in non-small cell lung cancer(NSCLC),and their relationship with clinicopathological parameters of NSCLC.[Methods]A total of 669 NSCLC patients who underwent surgical treatment in the Affiliated Hospital of Xuzhou Medical University from January 2017 to December 2018 were enrolled.The tumor tissues were collected for tissue microarray examination.The expression levels of PD-L1,TIL and FoxP3^(+)Treg were detected by immunohistochemical EnVision method.[Results]The expression of TIL was associated with gender,smoking history,histological types,lymph node metastasis,vascular invasion,and TNM staging in NSCLC patients(P<0.01).While the expression PD-L1 and FoxP3^(+)Treg was not associated with the clinicopathological features.PD-L1 and FoxP3^(+)Treg was positively correlated with TIL(P<0.01).Patients with low tumor differentiation,tumor>3 cm,lymph node metastasis,vascular invasion,TNMⅢ/Ⅳ,high PD-L1 expression and high FoxP3^(+)Treg expression had shorter recurrence-free survival(P<0.05).Patients with squamous cell carcinomas,male gender,smoking,low tumor differentiation,tumor>3 cm,lymph node metastasis,vascular invasion,TNMⅢ/Ⅳand low TIL infiltration had shorter overall survival.[Conclusion]The NSCLC patients with high expression of PD-L1 and FoxP3^(+)Treg and low lymphocyte infiltration have a poor prognosis.PD-L1 and FoxP3^(+)Treg can be used as independent prognostic indicators for postoperative recurrence/metastasis of NSCLC.
作者
沙梦玲
巩玉森
SHA Meng-ling;GONG Yu-sen(Affiliated Hospital of Xuzhou Medical University,Xuzhou 221006,China)
出处
《肿瘤学杂志》
CAS
2021年第1期36-41,共6页
Journal of Chinese Oncology
基金
徐州市科技计划项目(KC15SH043)。
作者简介
通信作者:巩玉森,副主任,本科,徐州医科大学附属医院病理科,江苏省徐州市泉山区淮海西路99号(221006),E-mail:66647465@qq.com。
