摘要
目的建立酒萸肉的超高效液相色谱指纹图谱,并进行聚类分析和主成分分析。方法色谱柱为ACE3 C18-AR柱(100 mm×2.1 mm,3μm),流动相为乙腈-0.3%磷酸溶液(梯度洗脱),流速为0.2 m L/min,检测波长为254 nm,柱温为25℃,进样量为1μL。以马钱苷为参照峰绘制76批酒萸肉药材样品的指纹图谱,并与10批山萸肉药材样品的指纹图谱进行比较。采用Chempattern化学计量学软件进行相似度评价,并进行聚类分析和主成分分析。结果76批酒萸肉药材指纹图谱共确定7个共有峰、6种成分,分别为没食子酸、5-羟甲基糠醛、莫诺苷、马钱苷、獐牙菜苷、山茱萸新苷;76批酒萸肉药材样品中有60批次的相似度大于0.8;76批酒萸肉药材样品和10批山萸肉药材样品聚为两大类,两大类的5-羟甲基糠醛含量差异明显;经主成分分析,峰6和峰2均是导致药材样品质量差异的重要因素。结论所建立的超高效液相色谱指纹图谱可用于酒萸肉的质量评价。
Objective To establish the ultra-performance liquid chromatography(UPLC)fingerprint of wine-prepared Fructus Corni,and to conduct cluster analysis and principal component analysis(PCA).Methods The chromatographic column was ACE3 C18-AR column(100 mm×2.1 mm,3μm),the mobile phase was acetonitrile-0.3%phosphoric acid solution(gradient elution),the flow rate was 0.2 m L/min,the detection wavelength was 254 nm,the column temperature was 25℃,and the injection volume was 1μL.The UPLC fingerprints of 76 batches of wine-prepared Fructus Corni were drawn with loganin as reference peak and compared with those of 10 batches of Fructus Corni.The similarity evaluation was carried out by Chempatten chemometrics software,and the cluster analysis and PCA were conducted.Results Seven common peaks and six main effective components were identified in the fingerprints of 76 batches of wine-prepared Fructus Corni,which were gallic acid,5-hydroxymethylfurfural,morroniside,loganin,swertioside and cornuside.The similarity of 60 batches of 76 batches of wine-prepared Fructus Corni was more than 0.8.Totally 76 batches of wine-prepared Fructus Corni samples and 10 batches of Fructus Corni samples were grouped into two groups,and the content of 5-hydroxymethylfurfural in the two groups was significantly different.The results of PCA showed that peak 6 and peak 2 were the important reasons for the quality difference of samples.Conclusion The established HPLC fingerprint can be used to evaluate the quality of wine-prepared Fructus Corni.
作者
李永鹏
卜晨琛
宋霞
刘亚蓉
LI Yongpeng;BU Chenchen;SONG Xia;LIU Yarong(Qinghai Provincial Institutes for Drug Control·Qinghai Key Lab for Modernization Research of Traditional Chinese and Tibetan Medicine,Xining,Qinghai China 810000)
出处
《中国药业》
CAS
2021年第6期35-40,共6页
China Pharmaceuticals
基金
青海省科技计划项目[2017-ZJ-Y40,2018-ZJ-T06]。
作者简介
第一作者:李永鹏,男,大学本科,主管药师,研究方向为中药药品检验,(电子信箱)453460131@qq.com.
