摘要
目的:建立和评价膝骨关节炎(KOA)肾阳虚大鼠模型。方法:24只大鼠随机分成正常组、KOA组、KOA去势组、KOA腺嘌呤组,每组6只。Hulth法建立KOA模型,去势法与腺嘌呤灌胃建立肾阳虚模型。通过Mankin’s和OARSI评分评价KOA模型;通过肾阳虚症状体征评价表及甲状腺轴、性腺轴、肾上腺轴激素水平等评价肾阳虚模型。结果:KOA组、KOA去势组、KOA腺嘌呤组均符合KOA病理表现,OARSI分级分别为2级、3级和4级。与其余3组比较,KOA腺嘌呤组肾阳虚症状体征积分最高(P<0.01,P<0.05),体质量增长最少(P<0.05),肛温最低(P<0.05),T3、T4、FSH、LH、T水平最低(P<0.05),E2水平最高(P<0.05);各组间CORT水平差异无统计学意义。结论:相较于Hulth+去势法,Hulth+腺嘌呤灌胃是构建KOA肾阳虚病证结合模型的更适宜方法。
Objective:To establish and evaluate the rat model of knee osteoarthritis(KOA)with kidney yang deficiency.Methods:Twenty-four rats were randomly divided into normal group,KOA group,KOA castration group and KOA adenine group,with 6 rats in each group.The KOA model was established by Hulth method,and the kidney yang deficiency model was established by castration method and adenine gavage.The KOA model was evaluated by Mankin’s and OARSI scores;the kidney yang deficiency model was assessed by a form of symptoms and signs of kidney yang deficiency,thyroid axis hormone levels,gonadal axis hormone levels,adrenal axis hormone levels,etc..Results:The KOA group,KOA castration group,and KOA adenine group were all consistent with the pathological manifestations of KOA,with the OARSI classification standard of grade 2,grade 3 and grade 4,respectively.Compared with the other three groups,KOA adenine group had the highest score of symptoms and signs of kidney yang deficiency(P<0.01,P<0.05),the least weight gain(P<0.05),the lowest anal temperature(P<0.05),the lowest levels of T3,T4,FSH,LH and T,and the highest level of E2(P<0.05);there was no significant difference on the level of CORT among groups.Conclusion:Compared with Hulth plus castration,Hulth plus adenine gavage is a more suitable method for the establishment of KOA kidney yang deficiency model.
作者
周丽
宋威江
杜梦梦
陈光耀
罗静
陶庆文
ZHOU Li;SONG Wei-jiang;DU Meng-meng;CHEN Guang-yao;LUO Jing;TAO Qing-wen(Beijing University of Chinese Medicine,Beijing 100029,China;Peking University Third Hospital,Beijing 100191,China;China-Japan Friendship Hospital,Beijing 100029,China;Beijing Key Lab for Immune-Mediated Inflammatory Diseases,Beijing 100029,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2021年第2期719-722,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81804042)。
关键词
膝骨关节炎
肾阳虚
动物模型
腺嘌呤
造模方法
Knee osteoarthritis
Deficiency of kidney yang
Animal model
Adenine
Molding method
作者简介
通信作者:罗静,北京巿朝阳区樱花园东街2号中日友好医院中医风湿病科,邮编:100029,电话:010-84205067,E-mail:luojinggga-sina.com;通信作者:陶庆文,北京市朝阳区樱花园东街2号中日友好医院中医风湿病科,邮编:100029,电话:010-84205067,E-mail:taongel@sina.com。
